TY - JOUR
T1 - Novel human esophagus-specific gene C1orf10
T2 - CDNA cloning, gene structure, and frequent loss of expression in esophageal cancer
AU - Xu, Zhixiong
AU - Wang, Ming Rong
AU - Xu, Xin
AU - Cai, Yan
AU - Han, Ya Ling
AU - Wu, Kong Ming
AU - Wang, Jie
AU - Chen, Bao Sheng
AU - Wang, Xiu Qin
AU - Wu, Min
PY - 2000/11/1
Y1 - 2000/11/1
N2 - We have identified a novel human gene, designated C1orf10, using modified differential display PCR. The C1orf10 gene, which spans 5 kb in length, is composed of three exons. The deduced protein contains 495 amino acids with one transmembrane domain. The amino acid sequence of C1orf10 is characterized by the presence of a calcium-binding motif of about 90 amino acids at its N-terminal and a conserved consecutive repeat sequence of 60 amino acids that was identiffed previously only in bacterial ice nucleation proteins. In normal adult tissues, C1orf10 is highly expressed only in the esophagus and was undetectable in a total of 15 other tissues examined, suggesting its important role in esophageal cells. The expression of C1orf10 is either dramatically reduced or absent in esophageal cancer cell lines (3/3) as well as primary esophageal cancer tissues (35/37) compared with the corresponding normal esophageal mucosa. Using a radiation hybrid panel, C1orf10 was found to be located on chromosome 1q21. These findings suggest that expression of C1orf10 is unique to esophageal cells and that loss of its expression may play a role in the development of esophageal cancer. (C) 2000 Academic Press.
AB - We have identified a novel human gene, designated C1orf10, using modified differential display PCR. The C1orf10 gene, which spans 5 kb in length, is composed of three exons. The deduced protein contains 495 amino acids with one transmembrane domain. The amino acid sequence of C1orf10 is characterized by the presence of a calcium-binding motif of about 90 amino acids at its N-terminal and a conserved consecutive repeat sequence of 60 amino acids that was identiffed previously only in bacterial ice nucleation proteins. In normal adult tissues, C1orf10 is highly expressed only in the esophagus and was undetectable in a total of 15 other tissues examined, suggesting its important role in esophageal cells. The expression of C1orf10 is either dramatically reduced or absent in esophageal cancer cell lines (3/3) as well as primary esophageal cancer tissues (35/37) compared with the corresponding normal esophageal mucosa. Using a radiation hybrid panel, C1orf10 was found to be located on chromosome 1q21. These findings suggest that expression of C1orf10 is unique to esophageal cells and that loss of its expression may play a role in the development of esophageal cancer. (C) 2000 Academic Press.
UR - http://www.scopus.com/inward/record.url?scp=0034329414&partnerID=8YFLogxK
U2 - 10.1006/geno.2000.6344
DO - 10.1006/geno.2000.6344
M3 - Article
C2 - 11056050
AN - SCOPUS:0034329414
SN - 0888-7543
VL - 69
SP - 322
EP - 330
JO - Genomics
JF - Genomics
IS - 3
ER -