Novel human esophagus-specific gene C1orf10: CDNA cloning, gene structure, and frequent loss of expression in esophageal cancer

Zhixiong Xu, Ming Rong Wang, Xin Xu, Yan Cai, Ya Ling Han, Kong Ming Wu, Jie Wang, Bao Sheng Chen, Xiu Qin Wang, Min Wu

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

We have identified a novel human gene, designated C1orf10, using modified differential display PCR. The C1orf10 gene, which spans 5 kb in length, is composed of three exons. The deduced protein contains 495 amino acids with one transmembrane domain. The amino acid sequence of C1orf10 is characterized by the presence of a calcium-binding motif of about 90 amino acids at its N-terminal and a conserved consecutive repeat sequence of 60 amino acids that was identiffed previously only in bacterial ice nucleation proteins. In normal adult tissues, C1orf10 is highly expressed only in the esophagus and was undetectable in a total of 15 other tissues examined, suggesting its important role in esophageal cells. The expression of C1orf10 is either dramatically reduced or absent in esophageal cancer cell lines (3/3) as well as primary esophageal cancer tissues (35/37) compared with the corresponding normal esophageal mucosa. Using a radiation hybrid panel, C1orf10 was found to be located on chromosome 1q21. These findings suggest that expression of C1orf10 is unique to esophageal cells and that loss of its expression may play a role in the development of esophageal cancer. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)322-330
Number of pages9
JournalGenomics
Volume69
Issue number3
DOIs
StatePublished - Nov 1 2000
Externally publishedYes

Fingerprint

Dive into the research topics of 'Novel human esophagus-specific gene C1orf10: CDNA cloning, gene structure, and frequent loss of expression in esophageal cancer'. Together they form a unique fingerprint.

Cite this