Novel fluo-4 analogs for fluorescent calcium measurements

Vladimir V. Martin; Michael Beierlein; Josh L. Morgan; Anca Rothe; Kyle R. Gee

doi:10.1016/j.ceca.2004.05.002

Novel fluo-4 analogs for fluorescent calcium measurements

Vladimir V. Martin
, Michael Beierlein
, Josh L. Morgan
, Anca Rothe
, Kyle R. Gee

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase.

Original language	English
Pages (from-to)	509-514
Number of pages	6
Journal	Cell Calcium
Volume	36
Issue number	6
DOIs	https://doi.org/10.1016/j.ceca.2004.05.002
State	Published - Dec 2004

Keywords

Biotin
Ca
Dextran
Fluorescence
Neuron
Protein
Reactive
Streptavidin

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10.1016/j.ceca.2004.05.002

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title = "Novel fluo-4 analogs for fluorescent calcium measurements",

abstract = "We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase.",

keywords = "Biotin, Ca, Dextran, Fluorescence, Neuron, Protein, Reactive, Streptavidin",

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T1 - Novel fluo-4 analogs for fluorescent calcium measurements

AU - Martin, Vladimir V.

AU - Beierlein, Michael

AU - Morgan, Josh L.

AU - Rothe, Anca

AU - Gee, Kyle R.

PY - 2004/12

Y1 - 2004/12

N2 - We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase.

AB - We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase.

KW - Biotin

KW - Ca

KW - Dextran

KW - Fluorescence

KW - Neuron

KW - Protein

KW - Reactive

KW - Streptavidin

UR - https://www.scopus.com/pages/publications/5544322164

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DO - 10.1016/j.ceca.2004.05.002

M3 - Article

C2 - 15488600

AN - SCOPUS:5544322164

SN - 0143-4160

VL - 36

SP - 509

EP - 514

JO - Cell Calcium

JF - Cell Calcium

IS - 6

ER -

Novel fluo-4 analogs for fluorescent calcium measurements

Abstract

Keywords

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