Novel fluo-4 analogs for fluorescent calcium measurements

Vladimir V. Martin, Michael Beierlein, Josh L. Morgan, Anca Rothe, Kyle R. Gee

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase.

Original languageEnglish
Pages (from-to)509-514
Number of pages6
JournalCell Calcium
Issue number6
StatePublished - Dec 2004


  • Biotin
  • Ca
  • Dextran
  • Fluorescence
  • Neuron
  • Protein
  • Reactive
  • Streptavidin


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