Abstract
Notch controls cell fate by inhibiting cellular differentiation, presumably through activation of the transcriptional regulator human C promoter Binding Factor (CBF1), which transactivates the hairy and Enhancer of split (HES-1) gene. However, we describe constitutively active forms of Notch1, which inhibit muscle cell differentiation but do not interact with CBF1 or upregulate endogenous HES-1 expression. In addition, Jagged-Notch interactions that prevent the expression of muscle cell specific genes do not involve the upregulation of endogenous HES-1. In fact, exogenous expression of HES-1 in C2C12 myoblasts does not block myogenesis. Our data demonstrate the existence of a CBF1-independent pathway by which Notch inhibits differentiation. We therefore propose that Notch signaling activates at least two different pathways: one which involves CBF1 as an intermediate and one which does not.
| Original language | English |
|---|---|
| Pages (from-to) | 3765-3773 |
| Number of pages | 9 |
| Journal | Development |
| Volume | 122 |
| Issue number | 12 |
| State | Published - 1996 |
Keywords
- CBF1
- HES-1
- Mouse
- Muscle cell differentiation
- Myoblast
- Notch