Normal T lymphocytes can express two different T cell receptor β chains: Implications for the mechanism of allelic exclusion

Elisabetta Padovan, Claudia Giachino, Marina Cella, Salvatore Valitutti, Oreste Acuto, Antonio Lanzavecchia

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

We have examined the extent of allelic exclusion at the T cell receptor (TCR)β locus using monoclonal antibodies specific for Vβ products. A small proportion (-1%) of human peripheral blood T cells express two Vβ as determined by flow cytometric analysis, isolation of representative clones, and sequencing of the corresponding Vβ chains. Dual β T cells are present in both the CD45R0+ and CD45R0 subset. These results indicate that dual β expression is compatible with both central and peripheral selection. They also suggest that the substantial degree of TCRβ allelic exclusion is dependent only on asynchronous rearrangements at the β locus, whereas the role of the pre-TCR is limited to signaling the presence of at least one functional β protein.

Original languageEnglish
Pages (from-to)1587-1591
Number of pages5
JournalJournal of Experimental Medicine
Volume181
Issue number4
DOIs
StatePublished - Apr 1 1995
Externally publishedYes

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