Noninvasive measurement of myocardial activity concentrations and perfusion defect sizes in rats with a new small-animal positron emission tomograph

Takashi Kudo, Kazuki Fukuchi, Alexander J. Annala, Arion F. Chatziioannou, Vivekanand Allada, Magnus Dahlbom, Yuan Chuan Tai, Masayuki Inubushi, Sung Cheng Huang, Simon R. Cherry, Michael E. Phelps, Heinrich R. Schelbert

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64 Scopus citations

Abstract

Background - We explored the feasibility of measuring regional tracer activity concentrations and flow defects in myocardium of rats with a high spatial resolution small-animal PET system (microPET). Methods and Results - Myocardial images were obtained after intravenous 18F-fluorodeoxyglucose (18FDG) in 11 normal rats (group 1) and assembled into polar maps. Regional 18F activity concentrations were measured in 9 regions of interest and compared with tissue activity concentrations measured by well counting. In another 9 rats (group 2), myocardial perfusion images were acquired with 13N-ammonia at baseline and during coronary occlusion. On the polar maps recorded during coronary occlusion, the size of perfusion defects was measured as the myocardium with <50% of maximum activity and expressed as percent total myocardium and was correlated with the area at risk defined by postmortem staining. The diagnostic quality of 18FDG and 13N-ammonia microPET images was good to excellent; the images were easily assembled into polar maps. In group 1, regional 18F concentrations by microPET and postmortem were correlated linearly (r=0.99; P<0.01 for average and r=0.97; P<0.01 for regional concentrations). In group 2, perfusion defect sizes by microPET and postmortem were correlated linearly (P<0.01; r=0.93). Conclusions - The findings indicate the feasibility of noninvasive studies of the myocardium in rats with a dedicated small-animal PET-imaging device.

Original languageEnglish
Pages (from-to)118-123
Number of pages6
JournalCirculation
Volume106
Issue number1
DOIs
StatePublished - Jul 1 2002
Externally publishedYes

Keywords

  • Imaging
  • Myocardium
  • Perfusion

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    Kudo, T., Fukuchi, K., Annala, A. J., Chatziioannou, A. F., Allada, V., Dahlbom, M., Tai, Y. C., Inubushi, M., Huang, S. C., Cherry, S. R., Phelps, M. E., & Schelbert, H. R. (2002). Noninvasive measurement of myocardial activity concentrations and perfusion defect sizes in rats with a new small-animal positron emission tomograph. Circulation, 106(1), 118-123. https://doi.org/10.1161/01.CIR.0000020221.28996.78