Noninvasive fluorescence detection of hepatic and renal function

Richard B. Dorshow, Joseph E. Bugaj, B. Daniel Burleigh, James R. Duncan, Michael A. Johnson, William B. Jones

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

A noninvasive in vivo fluorescence detection scheme was employed to continuously monitor exogenous dye clearance from the vasculature. Differentiation between normal and impaired physiological function in a rat model was demonstrated for both liver and kidney. A fiber optic transmitted light from source to ear; a second fiber optic positioned near the ear transmitted the fluorescent light to a detector system. Two model dye systems were employed in this initial study. Indocyanine green, known to be exclusively cleared from the blood stream by the liver, was excited in vivo with laser light at 780 nm. The fluorescence signal was detected at 830 nm. A characteristic clearance curve of normal hepatic function was obtained. After a partial hepatectomy of the liver, the clearance curve was extended in time, as would be expected from reduced hepatic function. In addition, fluorescein labeled poly-D-lysine, a small polymer predominantly cleared from the blood stream by the kidney, was excited in vivo with laser light at 488 nm. The fluorescence signal was detected at 518 nm. A characteristic clearance curve of normal renal function was obtained. After a bilateral ligation of the kidneys, the clearance curve remained elevated and constant, indicating little if any clearance. Thus, the feasibility of a new noninvasive method for physiological function assessment was established.

Original languageEnglish
Pages (from-to)340-345
Number of pages6
JournalJournal of biomedical optics
Volume3
Issue number3
DOIs
StatePublished - Jan 1 1998

Keywords

  • Dyes
  • Fluorescence
  • Hepatic clearance
  • Indocyanine green
  • Near infrared radiation
  • Renal clearance

Fingerprint Dive into the research topics of 'Noninvasive fluorescence detection of hepatic and renal function'. Together they form a unique fingerprint.

  • Cite this

    Dorshow, R. B., Bugaj, J. E., Daniel Burleigh, B., Duncan, J. R., Johnson, M. A., & Jones, W. B. (1998). Noninvasive fluorescence detection of hepatic and renal function. Journal of biomedical optics, 3(3), 340-345. https://doi.org/10.1117/1.429854