Noninvasive detection of brainstem and spinal cord axonal degeneration in an amyotrophic lateral sclerosis mouse model

Joong Hee Kim, Tzy Haw Wu, Matthew D. Budde, Jin Moo Lee, Sheng Kwei Song

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Degeneration of motor neurons and their associated axons is a hallmark of amyotrophic lateral sclerosis, but reliable noninvasive lesion detection is lacking. In vivo diffusion tensor imaging was performed to evaluate neurodegeneration in the brainstem and cervical spinal cord of wild-type and G93A-SOD1 transgenic mice, an animal model of amyotrophic lateral sclerosis. A statistically significant reduction in the apparent diffusion coefficient was observed in the motor nuclei VII and XII of G93A-SOD1 transgenic mice relative to wild-type mice. No significant difference in diffusion anisotropy was observed in dorsal white or gray matter in cervical and lumbar segments of the spinal cord. In contrast, statistically significant decreases in axial diffusivity (diffusivity parallel to the axis of the spinal cord) and apparent diffusion coefficient were found in the ventrolateral white matter of G93A-SOD1 mice in both the cervical and lumbar spinal cord. The reduction in axial diffusivity, suggestive of axonal injury, in the white matter of the spinal cord of G93A-SOD1 mice was verified by immunostaining with nonphosphorylated neurofilament. The present study demonstrates that in vivo diffusion tensor imaging-derived axial diffusivity may be used to accurately evaluate axonal degeneration in an animal model of amyotrophic lateral sclerosis.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalNMR in biomedicine
Volume24
Issue number2
DOIs
StatePublished - Feb 2011

Keywords

  • Amyotrophic lateral sclerosis
  • Axial and radial diffusivity
  • Cervical spinal cord
  • Diffusion anisotropy
  • Diffusion tensor imaging
  • G93A-SOD1
  • Mice

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