Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platforms

Brooke Sayre-Chavez, Bridget Baxter, Corey D. Broeckling, María Muñoz-Amatriaín, Mark Manary, Elizabeth P. Ryan

Research output: Contribution to journalArticlepeer-review

Abstract

Legumes are global staple foods with multiple human health properties that merit detailed composition analysis in cooked forms. This study analyzed cowpea [Vigna unguiculata] (three varieties: Dagbantuya, Sangyi, and Tukara), pigeon pea [Cajanus cajan], and common bean [Phaseolus vulgaris] using two distinct ultra-performance liquid chromatography mass spectrometry (UPLC-MS) platforms and analytical workflows. Comparisons between cowpea and pigeon pea consumed in Ghana, and common bean (navy bean) from USA, revealed 75 metabolites that differentiated cowpeas. Metabolite fold-change comparisons resulted in 142 metabolites with significantly higher abundance in cowpea, and 154 higher in abundance from pigeon pea. 3-(all-trans-nonaprenyl)benzene-1,2-diol, N-tetracosanoylphytosphingosine, and sitoindoside II are novel identifications in cowpea, with notably higher abundance than other legumes tested. Cowpea variety specific markers were tonkinelin (Dagbantuya), pheophytin A (Sangyi), and linoleoyl ethanolamide (Tukara). This study identified novel cowpea and pigeon pea food metabolites that warrant continued investigation as bioactive food components following consumption in people.

Original languageEnglish
Article number100087
JournalFood Chemistry: Molecular Sciences
Volume4
DOIs
StatePublished - Jul 30 2022

Keywords

  • 3-(all-trans-nonaprenyl)benzene-1,2-diol (PubChem CID: 25245248)
  • Cajanus cajan
  • Legumes
  • Linoleoyl ethanolamide (PubChem CID: 5283446)
  • N-tetracosanoylphytosphingosine (PubChem CID: 14653935)
  • Non-targeted metabolomics
  • Phaseolus vulgaris
  • Pheophytin A (PubChem CID: 135398712)
  • Phytochemicals
  • Pipecolic acid (PubChem CID: 849)
  • Piptamine (PubChem CID: 10664275)
  • Sitoindoside II (PubChem CID: 131751526)
  • Tonkinelin (PubChem CID: 73088078)
  • UPLC-MS
  • Vigna unguiculata

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