TY - JOUR
T1 - Non-targeted metabolomics of cooked cowpea (Vigna unguiculata) and pigeon pea (Cajanus cajan) from Ghana using two distinct and complementary analytical platforms
AU - Sayre-Chavez, Brooke
AU - Baxter, Bridget
AU - Broeckling, Corey D.
AU - Muñoz-Amatriaín, María
AU - Manary, Mark
AU - Ryan, Elizabeth P.
N1 - Funding Information:
This work was funded by the United States Agency for International Development (USAID) under Agreement No. 7200AA18LE00003 as part of Feed the Future Innovation Lab for Legume Systems Research. Any opinions, findings, conclusions, or recommendations expressed here are those of the authors alone.
Publisher Copyright:
© 2022
PY - 2022/7/30
Y1 - 2022/7/30
N2 - Legumes are global staple foods with multiple human health properties that merit detailed composition analysis in cooked forms. This study analyzed cowpea [Vigna unguiculata] (three varieties: Dagbantuya, Sangyi, and Tukara), pigeon pea [Cajanus cajan], and common bean [Phaseolus vulgaris] using two distinct ultra-performance liquid chromatography mass spectrometry (UPLC-MS) platforms and analytical workflows. Comparisons between cowpea and pigeon pea consumed in Ghana, and common bean (navy bean) from USA, revealed 75 metabolites that differentiated cowpeas. Metabolite fold-change comparisons resulted in 142 metabolites with significantly higher abundance in cowpea, and 154 higher in abundance from pigeon pea. 3-(all-trans-nonaprenyl)benzene-1,2-diol, N-tetracosanoylphytosphingosine, and sitoindoside II are novel identifications in cowpea, with notably higher abundance than other legumes tested. Cowpea variety specific markers were tonkinelin (Dagbantuya), pheophytin A (Sangyi), and linoleoyl ethanolamide (Tukara). This study identified novel cowpea and pigeon pea food metabolites that warrant continued investigation as bioactive food components following consumption in people.
AB - Legumes are global staple foods with multiple human health properties that merit detailed composition analysis in cooked forms. This study analyzed cowpea [Vigna unguiculata] (three varieties: Dagbantuya, Sangyi, and Tukara), pigeon pea [Cajanus cajan], and common bean [Phaseolus vulgaris] using two distinct ultra-performance liquid chromatography mass spectrometry (UPLC-MS) platforms and analytical workflows. Comparisons between cowpea and pigeon pea consumed in Ghana, and common bean (navy bean) from USA, revealed 75 metabolites that differentiated cowpeas. Metabolite fold-change comparisons resulted in 142 metabolites with significantly higher abundance in cowpea, and 154 higher in abundance from pigeon pea. 3-(all-trans-nonaprenyl)benzene-1,2-diol, N-tetracosanoylphytosphingosine, and sitoindoside II are novel identifications in cowpea, with notably higher abundance than other legumes tested. Cowpea variety specific markers were tonkinelin (Dagbantuya), pheophytin A (Sangyi), and linoleoyl ethanolamide (Tukara). This study identified novel cowpea and pigeon pea food metabolites that warrant continued investigation as bioactive food components following consumption in people.
KW - 3-(all-trans-nonaprenyl)benzene-1,2-diol (PubChem CID: 25245248)
KW - Cajanus cajan
KW - Legumes
KW - Linoleoyl ethanolamide (PubChem CID: 5283446)
KW - N-tetracosanoylphytosphingosine (PubChem CID: 14653935)
KW - Non-targeted metabolomics
KW - Phaseolus vulgaris
KW - Pheophytin A (PubChem CID: 135398712)
KW - Phytochemicals
KW - Pipecolic acid (PubChem CID: 849)
KW - Piptamine (PubChem CID: 10664275)
KW - Sitoindoside II (PubChem CID: 131751526)
KW - Tonkinelin (PubChem CID: 73088078)
KW - UPLC-MS
KW - Vigna unguiculata
UR - http://www.scopus.com/inward/record.url?scp=85125866213&partnerID=8YFLogxK
U2 - 10.1016/j.fochms.2022.100087
DO - 10.1016/j.fochms.2022.100087
M3 - Article
C2 - 35415674
AN - SCOPUS:85125866213
VL - 4
JO - Food Chemistry: Molecular Sciences
JF - Food Chemistry: Molecular Sciences
SN - 2666-5662
M1 - 100087
ER -