TY - JOUR
T1 - Non-O157:H7 pathogenic shiga toxin-producing Escherichia coli
T2 - Phenotypic and genetic profiling of virulence traits and evidence for clonality
AU - Schmidt, Herbert
AU - Geitz, Caren
AU - Tarr, Phillip I.
AU - Frosch, Matthias
AU - Karch, Helge
N1 - Funding Information:
Financial support: Deutsche Forschungsgemeinschaft (Sonderforsch-ungsbereich 479, “Erregervariabilität und Wirtsreaktion bei infektiösen Krankheitsprozessen”).
PY - 1999
Y1 - 1999
N2 - The virulence profiles of most non-O157 Shiga toxin (Stx)-producing Escherichia coli (STEC) are unknown. STEC belonging to serogroups O26, O103, and O111 were characterized to determine clonality and to profile virulence traits. Isolates within STEC serogroups O26, O111, and O103 were >80% identical in their randomly amplified polymorphic DNA pattern, suggesting clonality in these serogroups. The serogroups were distantly related to each other and to E. coli O157:H7. stx restriction fragment length polymorphism differentiated some STEC with the same randomly amplified polymorphic DNA pattern. The hemolytic phenotype of STEC O103:H2 isolates resembled that of α- and not enterohemorrhagic E. coli hemolysin. Virulence gene (eae, E-hly, espP, etp, katP, stx) probing demonstrated serogroup-specific profiles. Pathogenic STEC O26, O103, and O111 belong to their own lineages and have unique profiles of virulence traits that are different from the virulence profile of E. coli O157:H7.
AB - The virulence profiles of most non-O157 Shiga toxin (Stx)-producing Escherichia coli (STEC) are unknown. STEC belonging to serogroups O26, O103, and O111 were characterized to determine clonality and to profile virulence traits. Isolates within STEC serogroups O26, O111, and O103 were >80% identical in their randomly amplified polymorphic DNA pattern, suggesting clonality in these serogroups. The serogroups were distantly related to each other and to E. coli O157:H7. stx restriction fragment length polymorphism differentiated some STEC with the same randomly amplified polymorphic DNA pattern. The hemolytic phenotype of STEC O103:H2 isolates resembled that of α- and not enterohemorrhagic E. coli hemolysin. Virulence gene (eae, E-hly, espP, etp, katP, stx) probing demonstrated serogroup-specific profiles. Pathogenic STEC O26, O103, and O111 belong to their own lineages and have unique profiles of virulence traits that are different from the virulence profile of E. coli O157:H7.
UR - http://www.scopus.com/inward/record.url?scp=0032906605&partnerID=8YFLogxK
U2 - 10.1086/314537
DO - 10.1086/314537
M3 - Article
C2 - 9841830
AN - SCOPUS:0032906605
SN - 0022-1899
VL - 179
SP - 115
EP - 123
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -