Non-microfluidic methods for imaging live C. elegans

Cliff J. Luke, Jason Z. Niehaus, Linda P. O'Reilly, Simon C. Watkins

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

There are many challenges to live Caenorhabditis elegans imaging including the high motility of the animals and sustaining their viability for extended periods of time. Commonly used anesthetics to immobilize the C. elegans for imaging purpose prevents feeding of the animals and can cause cellular physiologic changes. Here we present three adapted or novel methodologies to image live C. elegans over different imaging microscopy equipment to allow for visualization of animals by DIC and fluorescence without the use of microfluidic technologies. The methods present here use common microscopy consumables and equipment found in many imaging core facilities and can be easily adapted to fit on multiple microscopy systems.

Original languageEnglish
Pages (from-to)542-547
Number of pages6
JournalMethods
Volume68
Issue number3
DOIs
StatePublished - Aug 1 2014
Externally publishedYes

Keywords

  • Caenorhabditis elegans
  • Confocal
  • Fluorescence
  • Live-cell imaging
  • Microscopy
  • Widefield

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