Noda-like RNA viruses infecting Caenorhabditis nematodes: Sympatry, diversity, and reassortment

Lise Frézal, Hyeim Jung, Stephen Tahan, David Wang, Marie Anne Félix

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Three RNA viruses related to nodaviruses were previously described to naturally infect the nematode Caenorhabditis elegans and its relative, Caenorhabditis briggsae. Here, we report on a collection of more than 50 viral variants from wild-caught Caenorhabditis. We describe the discovery of a new related virus, the Mělník virus, infecting C. briggsae, which similarly infects intestinal cells. In France, a frequent pattern of coinfection of C. briggsae by the Santeuil virus and Le Blanc virus was observed at the level of an individual nematode and even a single cell. We do not find evidence of reassortment between the RNA1 and RNA2 molecules of Santeuil and Le Blanc viruses. However, by studying patterns of evolution of each virus, reassortments of RNA1 and RNA2 among variants of each virus were identified. We develop assays to test the relative infectivity and competitive ability of the viral variants and detect an interaction between host genotype and Santeuil virus genotype, such that the result depends on the host strain. IMPORTANCE The roundworm Caenorhabditis elegans is a laboratory model organism in biology. We study natural populations of this small animal and its relative, C. briggsae, and the viruses that infect them. We previously discovered three RNA viruses related to nodaviruses and here describe a fourth one, called the Mělník virus. These viruses have a genome composed of two RNA molecules. We find that two viruses may infect the same animal and the same cell. The two RNA molecules may be exchanged between variants of a given viral species. We study the diversity of each viral species and devise an assay of their infectivity and competitive ability. Using this assay, we show that the outcome of the competition also depends on the host.

Original languageEnglish
Article numbere01170-19
JournalJournal of virology
Volume93
Issue number21
DOIs
StatePublished - 2019

Keywords

  • C. briggsae
  • C. elegans
  • Coinfection
  • Genetic diversity
  • Le Blanc virus
  • Mělník virus
  • Nodavirus
  • Orsay virus
  • Santeuil virus
  • Viral RNA reassortment
  • Viral competition

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