N.m.r. studies of metabolism in perfused organs.

J. J. Ackerman, P. J. Bore, D. G. Gadian, T. H. Grove, G. K. Radda

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Several metabolites and intracellular pH in intact organs can be studied in a non-destructive manner by phorphorus nuclear magnetic resonance (31P n.m.r.). This possibility was demonstrated by us nearly five years ago. Since then we have developed the appropriate physiological techniques and improved the n.m.r. method for the study of animal hearts and kidneys. Here we described measurements aimed at clarifying three problesm. (1) Having measured the enzyme-catalysed fluxes between phosphocreatine and ATP by the method of saturation transfer n.m.r., we examine the relations between energy supply and heart rate in the isolated perfused rat heart. (2) We describe experiments to establish the validity of the perfusion model. For the first time, we report 31P n.m.r. measurements of an in vivo rat heart and compare the results with those obtained for the perfused rat heart. (3) Ischaemia and metabolism in rabbit kidneys is investigated to establish the relation between functional and metabolic recovery after a renal transplant operation.

Original languageEnglish
Pages (from-to)425-436
Number of pages12
JournalPhilosophical transactions of the Royal Society of London. Series B, Biological sciences
Volume289
Issue number1037
DOIs
StatePublished - Jun 25 1980
Externally publishedYes

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