NLRP6 inflammasome regulates colonic microbial ecology and risk for colitis

Eran Elinav, Till Strowig, Andrew L. Kau, Jorge Henao-Mejia, Christoph A. Thaiss, Carmen J. Booth, David R. Peaper, John Bertin, Stephanie C. Eisenbarth, Jeffrey I. Gordon, Richard A. Flavell

Research output: Contribution to journalArticlepeer-review

1410 Scopus citations

Abstract

Inflammasomes are multiprotein complexes that function as sensors of endogenous or exogenous damage-associated molecular patterns. Here, we show that deficiency of NLRP6 in mouse colonic epithelial cells results in reduced IL-18 levels and altered fecal microbiota characterized by expanded representation of the bacterial phyla Bacteroidetes (Prevotellaceae) and TM7. NLRP6 inflammasome-deficient mice were characterized by spontaneous intestinal hyperplasia, inflammatory cell recruitment, and exacerbation of chemical colitis induced by exposure to dextran sodium sulfate (DSS). Cross-fostering and cohousing experiments revealed that the colitogenic activity of this microbiota is transferable to neonatal or adult wild-type mice, leading to exacerbation of DSS colitis via induction of the cytokine, CCL5. Antibiotic treatment and electron microscopy studies further supported the role of Prevotellaceae as a key representative of this microbiota-associated phenotype. Altogether, perturbations in this inflammasome pathway, including NLRP6, ASC, caspase-1, and IL-18, may constitute a predisposing or initiating event in some cases of human IBD. PaperClip:

Original languageEnglish
Pages (from-to)745-757
Number of pages13
JournalCell
Volume145
Issue number5
DOIs
StatePublished - May 27 2011

Fingerprint

Dive into the research topics of 'NLRP6 inflammasome regulates colonic microbial ecology and risk for colitis'. Together they form a unique fingerprint.

Cite this