NK and CTL recognition of a single chain H-2D(d) molecule: Distinct sites of H-2D(d) interact with NK and TCR

Doo Hyun Chung, Jeffrey Dorfman, Daniel Plaksin, Kannan Natarajan, Igor M. Belyakov, Rosemarie Hunziker, Jay A. Berzofsky, Wayne M. Yokoyama, Michael G. Mage, David H. Margulies

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Abstract

We generated transgenic mice expressing a single-chain β2- microglobulin (β2m)-H-2D(d). The cell-surface-β2m-H-2D(d) molecule was expressed on a β2m-deficient background and reacted with appropriate mAbs. It was of the expected m.w. and directed the normal development of CD8+ T cells in the thymus of a broad TCR repertoire. It also presented both exogenously provided and endogenous peptide Ags to effector CD8+ T cells. In tests of NK cell education and function, it failed to reveal any interaction with NK cells, suggesting that the site of the interaction of NK receptors with H-2D(d) was disrupted. Thus, the sites of TCR and NK receptor interaction with H-2D(d) are distinct, an observation consistent with independent modes of TCR and NK receptor evolution and function.

Original languageEnglish
Pages (from-to)3699-3708
Number of pages10
JournalJournal of Immunology
Volume163
Issue number7
StatePublished - Oct 1 1999

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    Chung, D. H., Dorfman, J., Plaksin, D., Natarajan, K., Belyakov, I. M., Hunziker, R., Berzofsky, J. A., Yokoyama, W. M., Mage, M. G., & Margulies, D. H. (1999). NK and CTL recognition of a single chain H-2D(d) molecule: Distinct sites of H-2D(d) interact with NK and TCR. Journal of Immunology, 163(7), 3699-3708.