We generated transgenic mice expressing a single-chain β2- microglobulin (β2m)-H-2D(d). The cell-surface-β2m-H-2D(d) molecule was expressed on a β2m-deficient background and reacted with appropriate mAbs. It was of the expected m.w. and directed the normal development of CD8+ T cells in the thymus of a broad TCR repertoire. It also presented both exogenously provided and endogenous peptide Ags to effector CD8+ T cells. In tests of NK cell education and function, it failed to reveal any interaction with NK cells, suggesting that the site of the interaction of NK receptors with H-2D(d) was disrupted. Thus, the sites of TCR and NK receptor interaction with H-2D(d) are distinct, an observation consistent with independent modes of TCR and NK receptor evolution and function.
|Number of pages||10|
|Journal||Journal of Immunology|
|State||Published - Oct 1 1999|