TY - JOUR
T1 - Nitric oxide inhibitors attenuate ischemic degeneration in the CA1 region of rat hippocampal slices
AU - Izumi, Y.
AU - Benz, A. M.
AU - Clifford, D. B.
AU - Zorumski, C. F.
PY - 1996
Y1 - 1996
N2 - We examined the involvement of nitric oxide (NO) in ischemic brain damage using hippocampal slices prepared from 30 day old albino rats and exposed to 20 min of oxygen/glucose deprivation (ischemia) followed by 90 min postincubation in oxygen- and glucose-containing media. Damage in the CA1 region was rated on a 0 (intact) to 4 (severe neuronal damage) scale by a rater blind to the experimental condition. Control slices exposed to ischemia were rated as 2.8 ± 0.4 (N = 12); L-N(G)-Monomethylarginine and L-N(G)-nitroarginine (100 μM), non-selective NO synthase (NOS) inhibitors, diminished ischemic damage (0.6 ± 0.3, N = 8, and 1.0 ± 0.5, N = 4, respectively). An inhibitor of brain NOS, 7-nitroindazole (30 μM), was also effective-against ischemic degeneration (0.7 ± 0.3, N = 5). These results suggest that activation of NOS is involved in ischemic degeneration in the CA1 region.
AB - We examined the involvement of nitric oxide (NO) in ischemic brain damage using hippocampal slices prepared from 30 day old albino rats and exposed to 20 min of oxygen/glucose deprivation (ischemia) followed by 90 min postincubation in oxygen- and glucose-containing media. Damage in the CA1 region was rated on a 0 (intact) to 4 (severe neuronal damage) scale by a rater blind to the experimental condition. Control slices exposed to ischemia were rated as 2.8 ± 0.4 (N = 12); L-N(G)-Monomethylarginine and L-N(G)-nitroarginine (100 μM), non-selective NO synthase (NOS) inhibitors, diminished ischemic damage (0.6 ± 0.3, N = 8, and 1.0 ± 0.5, N = 4, respectively). An inhibitor of brain NOS, 7-nitroindazole (30 μM), was also effective-against ischemic degeneration (0.7 ± 0.3, N = 5). These results suggest that activation of NOS is involved in ischemic degeneration in the CA1 region.
KW - 7-Nitroindazole
KW - Cerebral ischemia
KW - Excitotoxicity
KW - Hippocampal slices
KW - N-Methyl-D-aspartate
KW - Nitric oxide synthase inhibitor
UR - http://www.scopus.com/inward/record.url?scp=0029975274&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(96)12669-0
DO - 10.1016/0304-3940(96)12669-0
M3 - Article
C2 - 8805119
AN - SCOPUS:0029975274
SN - 0304-3940
VL - 210
SP - 157
EP - 160
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -