TY - JOUR
T1 - NFIX regulates proliferation and migration within the murine SVZ neurogenic niche
AU - Evelyn Heng, Yee Hsieh
AU - Zhou, Bo
AU - Harris, Lachlan
AU - Harvey, Tracey
AU - Smith, Aaron
AU - Horne, Elise
AU - Martynoga, Ben
AU - Andersen, Jimena
AU - Achimastou, Angeliki
AU - Cato, Kathleen
AU - Richards, Linda J.
AU - Gronostajski, Richard M.
AU - Yeo, Giles S.
AU - Guillemot, François
AU - Bailey, Timothy L.
AU - Piper, Michael
N1 - Publisher Copyright:
© 2014 The Author.
PY - 2015/10
Y1 - 2015/10
N2 - Transcription factors of the nuclear factor one (NFI) family play a pivotal role in the development of the nervous system. One member, NFIX, regulates the development of the neocortex, hippocampus, and cerebellum. Postnatal Nfix-/- mice also display abnormalities within the subventricular zone (SVZ) lining the lateral ventricles, a region of the brain comprising a neurogenic niche that provides ongoing neurogenesis throughout life. Specifically, Nfix-/- mice exhibit more PAX6-expressing progenitor cells within the SVZ. However, the mechanism underlying the development of this phenotype remains undefined. Here, we reveal that NFIX contributes to multiple facets of SVZ development. Postnatal Nfix-/- mice exhibit increased levels of proliferation within the SVZ, both in vivo and in vitro as assessed by a neurosphere assay. Furthermore, we show that the migration of SVZ-derived neuroblasts to the olfactory bulb is impaired, and that the olfactory bulbs of postnatal Nfix-/- mice are smaller.We also demonstrate that gliogenesis within the rostral migratory stream is delayed in the absence of Nfix, and reveal that Gdnf (glial-derived neurotrophic factor), a known attractant for SVZ-derived neuroblasts, is a target for transcriptional activation by NFIX. Collectively, these findings suggest that NFIX regulates both proliferation and migration during the development of the SVZ neurogenic niche.
AB - Transcription factors of the nuclear factor one (NFI) family play a pivotal role in the development of the nervous system. One member, NFIX, regulates the development of the neocortex, hippocampus, and cerebellum. Postnatal Nfix-/- mice also display abnormalities within the subventricular zone (SVZ) lining the lateral ventricles, a region of the brain comprising a neurogenic niche that provides ongoing neurogenesis throughout life. Specifically, Nfix-/- mice exhibit more PAX6-expressing progenitor cells within the SVZ. However, the mechanism underlying the development of this phenotype remains undefined. Here, we reveal that NFIX contributes to multiple facets of SVZ development. Postnatal Nfix-/- mice exhibit increased levels of proliferation within the SVZ, both in vivo and in vitro as assessed by a neurosphere assay. Furthermore, we show that the migration of SVZ-derived neuroblasts to the olfactory bulb is impaired, and that the olfactory bulbs of postnatal Nfix-/- mice are smaller.We also demonstrate that gliogenesis within the rostral migratory stream is delayed in the absence of Nfix, and reveal that Gdnf (glial-derived neurotrophic factor), a known attractant for SVZ-derived neuroblasts, is a target for transcriptional activation by NFIX. Collectively, these findings suggest that NFIX regulates both proliferation and migration during the development of the SVZ neurogenic niche.
KW - Neuroblast
KW - Nuclear factor one X
KW - Olfactory bulb
KW - Rostral migratory stream
KW - Subventricular zone
UR - http://www.scopus.com/inward/record.url?scp=84948665083&partnerID=8YFLogxK
U2 - 10.1093/cercor/bhu253
DO - 10.1093/cercor/bhu253
M3 - Article
C2 - 25331604
AN - SCOPUS:84948665083
SN - 1047-3211
VL - 25
SP - 3758
EP - 3778
JO - Cerebral Cortex
JF - Cerebral Cortex
IS - 10
ER -