TY - JOUR
T1 - Next-Generation Serology by Mass Spectrometry
T2 - Readout of the SARS-CoV-2 Antibody Repertoire
AU - Melani, Rafael D.
AU - Des Soye, Benjamin J.
AU - Kafader, Jared O.
AU - Forte, Eleonora
AU - Hollas, Michael
AU - Blagojevic, Voislav
AU - Negrão, Fernanda
AU - McGee, John P.
AU - Drown, Bryon
AU - Lloyd-Jones, Cameron
AU - Seckler, Henrique S.
AU - Camarillo, Jeannie M.
AU - Compton, Philip D.
AU - LeDuc, Richard D.
AU - Early, Bryan
AU - Fellers, Ryan T.
AU - Cho, Byoung Kyu
AU - Mattamana, Basil Baby
AU - Goo, Young Ah
AU - Thomas, Paul M.
AU - Ash, Michelle K.
AU - Bhimalli, Pavan P.
AU - Al-Harthi, Lena
AU - Sha, Beverly E.
AU - Schneider, Jeffrey R.
AU - Kelleher, Neil L.
N1 - Publisher Copyright:
© 2021 American Chemical Society
PY - 2022/1/7
Y1 - 2022/1/7
N2 - Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses recent advances in protein mass spectrometry (MS) for multiparametric readout of antibodies, with new metrics like Ion Titer (IT) and Degree of Clonality (DoC) capturing the heterogeneity and relative abundance of individual clones without sequencing of B cells. We applied Ig-MS to plasma from subjects with severe and mild COVID-19 and immunized subjects after two vaccine doses, using the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 as the bait for antibody capture. Importantly, we report a new data type for human serology, that could use other antigens of interest to gauge immune responses to vaccination, pathogens, or autoimmune disorders.
AB - Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses recent advances in protein mass spectrometry (MS) for multiparametric readout of antibodies, with new metrics like Ion Titer (IT) and Degree of Clonality (DoC) capturing the heterogeneity and relative abundance of individual clones without sequencing of B cells. We applied Ig-MS to plasma from subjects with severe and mild COVID-19 and immunized subjects after two vaccine doses, using the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 as the bait for antibody capture. Importantly, we report a new data type for human serology, that could use other antigens of interest to gauge immune responses to vaccination, pathogens, or autoimmune disorders.
KW - COVID-19
KW - SARS-CoV-2
KW - antibodies
KW - individual ion mass spectrometry
KW - proteomics
KW - serology
KW - top-down mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=85121237927&partnerID=8YFLogxK
U2 - 10.1021/acs.jproteome.1c00882
DO - 10.1021/acs.jproteome.1c00882
M3 - Article
C2 - 34878788
AN - SCOPUS:85121237927
SN - 1535-3893
VL - 21
SP - 274
EP - 288
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 1
ER -