TY - JOUR
T1 - New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide
AU - Casabuono, Adriana C.
AU - Czibener, Cecilia
AU - Del Giudice, Mariela G.
AU - Valguarnera, Ezequiel
AU - Ugalde, Juan E.
AU - Couto, Alicia S.
N1 - Funding Information:
This work was supported by the Consejo Nacional de Investigaciones Científicas y Técnicas Grant PIP-11220110100660, Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) grant PICT-2013-0736 and PICT-2014-1028 and Universidad de Buenos Aires grant 20020130100476BA. The Ultraflex II (Bruker) TOF/TOF mass spectrometer was supported by ANPCyT grant PME 125 (CEQUIBIEM). A.S.C., J.E.U., and C.C. are members of Carrera de Investigador Científico y Tecnológico of CONICET.
Publisher Copyright:
© 2017, American Society for Mass Spectrometry.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Brucellaceae are Gram-negative bacteria that cause brucellosis, one of the most distributed worldwide zoonosis, transmitted to humans by contact with either infected animals or their products. The lipopolysaccharide exposed on the cell surface has been intensively studied and is considered a major virulence factor of Brucella. In the last years, structural studies allowed the determination of new structures in the core oligosaccharide and the O-antigen of this lipopolysaccharide. In this work, we have reinvestigated the lipid A structure isolated from B. suis and B. abortus lipopolysaccharides. A detailed study by MALDI-TOF mass spectrometry in the positive and negative ion modes of the lipid A moieties purified from both species was performed. Interestingly, a new feature was detected: the presence of a pyrophosphorylethanolamine residue substituting the backbone. LID-MS/MS analysis of some of the detected ions allowed assurance that the Lipid A structure composed by the diGlcN3N disaccharide, mainly hexa-acylated and penta-acylated, bearing one phosphate and one pyrophosphorylethanolamine residue. [Figure not available: see fulltext.].
AB - Brucellaceae are Gram-negative bacteria that cause brucellosis, one of the most distributed worldwide zoonosis, transmitted to humans by contact with either infected animals or their products. The lipopolysaccharide exposed on the cell surface has been intensively studied and is considered a major virulence factor of Brucella. In the last years, structural studies allowed the determination of new structures in the core oligosaccharide and the O-antigen of this lipopolysaccharide. In this work, we have reinvestigated the lipid A structure isolated from B. suis and B. abortus lipopolysaccharides. A detailed study by MALDI-TOF mass spectrometry in the positive and negative ion modes of the lipid A moieties purified from both species was performed. Interestingly, a new feature was detected: the presence of a pyrophosphorylethanolamine residue substituting the backbone. LID-MS/MS analysis of some of the detected ions allowed assurance that the Lipid A structure composed by the diGlcN3N disaccharide, mainly hexa-acylated and penta-acylated, bearing one phosphate and one pyrophosphorylethanolamine residue. [Figure not available: see fulltext.].
KW - Brucella
KW - Lipid A
KW - Lipopolysaccharide
KW - MALDI-TOF MS
UR - http://www.scopus.com/inward/record.url?scp=85037042247&partnerID=8YFLogxK
U2 - 10.1007/s13361-017-1805-x
DO - 10.1007/s13361-017-1805-x
M3 - Article
C2 - 28924631
AN - SCOPUS:85037042247
SN - 1044-0305
VL - 28
SP - 2716
EP - 2723
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 12
ER -