New approach to optical imaging of tumors

S. Achilefu, J. E. Bugaj, R. B. Dorshow, H. N. Jimenez, R. Rajagopalan

Research output: Contribution to journalConference articlepeer-review

10 Scopus citations

Abstract

Site-specific delivery of drugs and contrast agents to tumors protects normal tissues from the cytotoxic effect of drugs, and enhances the contrast between normal and diseased tissues. In optical medicine, biocompatible dyes can be used as phototherapeutics or as contrast agents. Previous studies have shown that the use of covalent or non-covalent dye conjugates of carriers such as antibodies, liposomes, and polysaccharides improves the delivery of such molecules to tumors. However, large biomolecules can elicit adverse immunogenic reactions and also result in long blood clearance times, delaying visualization of target tissues. A viable alternative to this strategy is to use small bioactive molecule-dye conjugates. These molecules have several advantages over large biomolecules, including ease of synthesis of a variety of high purity compounds for combinatorial screening of new targets, enhanced diffusivity to solid tumors, and the ability to affect the pharmacokinetics of the conjugates by minor structural changes. Thus, we conjugated a near infrared absorbing dye to several bioactive peptides that specifically target overexpressed tumor receptors in established rat tumor lines. High tumor uptake of the conjugates was obtained without loss of either the peptide receptor affinity or the dye fluorescence. These findings demonstrate the efficacy of a small peptide-dye conjugate strategy for in vivo tumor imaging. Site-specific delivery of photodynamic therapy agents may also benefit from this approach.

Original languageEnglish
Pages (from-to)110-114
Number of pages5
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4259
DOIs
StatePublished - Jan 1 2001
EventBiomarkers and Biological Spectral Imaging - San Jose, CA, United States
Duration: Jan 23 2001 → …

Keywords

  • Contrast agents
  • Dyes
  • Fluorescence
  • Indocyanine green
  • Optical imaging
  • Peptide-dye conjugates
  • Photodiagnostics
  • Phototherapeutics
  • Targeting agents
  • Tumor detection

Fingerprint

Dive into the research topics of 'New approach to optical imaging of tumors'. Together they form a unique fingerprint.

Cite this