TY - JOUR
T1 - Neutrophils induce the maturation of immature dendritic cells
T2 - A regulatory role of neutrophils in adaptive immune responses
AU - Xiaoxiao, Wan
AU - Sibiao, Yue
AU - Xiaopeng, Xiong
AU - Ping, Zheng
AU - Gang, Chen
PY - 2007/5
Y1 - 2007/5
N2 - Th1-type immune cytokines are essential to establish adaptive immunity against various microbial pathogens, including Escherichia coli, which cause most urinary tract infections (UTIs). Dendritic cells (DCs) are vital to initiate Th1 immunity, while neutrophils, also referred to here as polymorphonuclear leukocytes (PMN) are reported to be involved in Th1 immunity initiation by secreting several chemokines and cytokines. We found that lipopolysaccharide (LPS)-triggered PMN (LPS-PMN) in vitro induced strong up-regulation of DCs surface markers CD40, CD80, MHC-II (Iab), and CD86 either by secreting soluble factors, such as TNF-α, or by PMN-DC cellular contact. LPS-PMN also stimulated DCs to produce IL-12 and TNF-α. Furthermore, purified DCs activated by LPS-PMN were able to present specific antigen to T cells and drive Th1 differentiation by producing large amount of IFN-γ but low amount of IL-4. Our results suggest a regulatory role of PMN for DCs function in adaptive immune responses, thereby providing a link between innate and adaptive immunity.
AB - Th1-type immune cytokines are essential to establish adaptive immunity against various microbial pathogens, including Escherichia coli, which cause most urinary tract infections (UTIs). Dendritic cells (DCs) are vital to initiate Th1 immunity, while neutrophils, also referred to here as polymorphonuclear leukocytes (PMN) are reported to be involved in Th1 immunity initiation by secreting several chemokines and cytokines. We found that lipopolysaccharide (LPS)-triggered PMN (LPS-PMN) in vitro induced strong up-regulation of DCs surface markers CD40, CD80, MHC-II (Iab), and CD86 either by secreting soluble factors, such as TNF-α, or by PMN-DC cellular contact. LPS-PMN also stimulated DCs to produce IL-12 and TNF-α. Furthermore, purified DCs activated by LPS-PMN were able to present specific antigen to T cells and drive Th1 differentiation by producing large amount of IFN-γ but low amount of IL-4. Our results suggest a regulatory role of PMN for DCs function in adaptive immune responses, thereby providing a link between innate and adaptive immunity.
KW - Adaptive immunity
KW - Dendritic cells
KW - Neutrophils
UR - http://www.scopus.com/inward/record.url?scp=34250158792&partnerID=8YFLogxK
U2 - 10.1080/08820130601109719
DO - 10.1080/08820130601109719
M3 - Article
C2 - 17558715
AN - SCOPUS:34250158792
SN - 0882-0139
VL - 36
SP - 337
EP - 350
JO - Immunological Investigations
JF - Immunological Investigations
IS - 3
ER -