Interleukin-12 (IL-12) is necessary for the production of IFN-γ by NK cells during the generation of innate immunity and by T cells for the development of the Th1 response during specific cell mediated immunity. Here we demonstrate that the endogenous production of IL-12 is critical to the survival of both immunocompromised SCID mice and normal C.B-17 control mice during a primary infection with Listeria monocytogenes. When IL-12 is neutralized in vivo, both strains of mice die at a normally sublethal dose of Listeria. Anti-IL-12 antibody-treated mice showed a decrease in macrophage I- A(d) expression and an increased Listeria burden in the spleen. Furthermore, as has been demonstrated in vitro, these effects of IL-12 in vivo were predominantly regulated through the production of IFN-γ. Administration of IFN-γ simultaneously with neutralizing antibodies to IL-12 restored macrophage I-A(d) expression, limited the spread of the infection, and resulted in the survival of SCID mice. Thus, IL-12 is critical for resistance to infection with Listeria monocytogenes, and this resistance is mediated through stimulation by IL-12 of IFN-γ production. Concomitant experiments confirmed that anti-TNF antibodies also resulted in uncontrolled infection and a decrease in macrophage I-A(d) expression. However, administration of IFN-γ restored the levels of I-A(d) in macrophages but did not limit Listeria growth.
|Number of pages||5|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1994|