@article{10a329a4732e48c586302bcf42310bfd,
title = "Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development",
abstract = "Neuropilin-1 (Npn-1) is a receptor that binds multiple ligands from structurally distinct families, including secreted semaphorins (Sema) and vascular endothelial growth factors (VEGF). We generated npn-1 knockin mice, which express an altered ligand binding site variant of Npn-1, and npn-1 conditional null mice to establish the cell-type- and ligand specificity of Npn-1 function in the developing cardiovascular and nervous systems. Our results show that VEGF-Npn-1 signaling in endothelial cells is required for angiogenesis. In striking contrast, Sema-Npn-1 signaling is not essential for general vascular development but is required for axonal pathfinding by several populations of neurons in the CNS and PNS. Remarkably, both Sema-Npn-1 signaling and VEGF-Npn-1 signaling are critical for heart development. Therefore, Npn-1 is a multifunctional receptor that mediates the activities of structurally distinct ligands during development of the heart, vasculature, and nervous system.",
author = "Chenghua Gu and Rodriguez, {E. Rene} and Reimert, {Dorothy V.} and Tianzhi Shu and Bernd Fritzsch and Richards, {Linda J.} and Kolodkin, {Alex L.} and Ginty, {David D.}",
note = "Funding Information: We thank members of the Kolodkin and Ginty laboratories, Jan Rosenbaum, Daniel Leahy, and Anirvan Ghosh for helpful discussions and comments on the manuscript. We thank Mitra Cowan and the Johns Hopkins Transgenic Facility for blastocyst injections and advice with ES cells, Guo Huang for help with the TrkA staining experiments, Sarah Lawson and Todd Hippe for excellent technical assistance, Naren Ramanan for help with preparation of the figures, Kogo Takamiya and Richard Huganir for advice with targeting vectors, Susan Dymecki for the germline FlpE mice, and Masashi Yanagisawa for the Tie-2-Cre mice. This work was supported by NIH/NRSA-5F32NS11016, NIH/NHLBI P01-HL70295, NIH/NIDCD R01-DC 005590, the Robert Packard Center for ALS Research at Johns Hopkins, NIH/NIMH-R01MH59199, the Kirsch Foundation, and the Howard Hughes Medical Institute.",
year = "2003",
month = jul,
day = "1",
doi = "10.1016/S1534-5807(03)00169-2",
language = "English",
volume = "5",
pages = "45--57",
journal = "Developmental cell",
issn = "1534-5807",
number = "1",
}