TY - JOUR
T1 - Neuronal Transcriptome of Aplysia
T2 - Neuronal Compartments and Circuitry
AU - Moroz, Leonid L.
AU - Edwards, John R.
AU - Puthanveettil, Sathyanarayanan V.
AU - Kohn, Andrea B.
AU - Ha, Thomas
AU - Heyland, Andreas
AU - Knudsen, Bjarne
AU - Sahni, Anuj
AU - Yu, Fahong
AU - Liu, Li
AU - Jezzini, Sami
AU - Lovell, Peter
AU - Iannucculli, William
AU - Chen, Minchen
AU - Nguyen, Tuan
AU - Sheng, Huitao
AU - Shaw, Regina
AU - Kalachikov, Sergey
AU - Panchin, Yuri V.
AU - Farmerie, William
AU - Russo, James J.
AU - Ju, Jingyue
AU - Kandel, Eric R.
N1 - Funding Information:
We thank Tom Carew, Kelsey Martin, Kausik Si, Tom Jessel, Wayne Sossin, John Byrne, and Larry Zipursky for their comments on earlier versions of this paper. Our work is supported by HHMI, NSF, and National Institutes of Health Center of Excellence in Genomic Science Grants P50 HG002806 and R01 MH075026, NS39103, and in part by the McKnight Brain Research Foundations, UF Opportunity Funds, and RFBR- 05-04-48401. We also would like to thank Mrs. E. Bobkova, T. Brough, E. Meleshkevitch, J. Netherton, and Drs. M. Matz, N. Alieva, and R. Sadreyev for technical help and comments at earlier stages of this project. We thank MOgene (LC), and in particular Shaukat Rangwala, for the microarray analysis, and Drs. E. Koonin and Y. Wolf for KOGs discussions. Computational work for this project was supported by The AMDeC Bioinformatics Core Facility at the Columbia Genome Center, Columbia University, and the ICBR Core facility at the University of Florida. Currently, Y. Panchin is at Moscow State University, Moscow, Russia; and B. Knudsen is at CLC bio A/S Aarhus, Denmark.
PY - 2006/12/29
Y1 - 2006/12/29
N2 - Molecular analyses of Aplysia, a well-established model organism for cellular and systems neural science, have been seriously handicapped by a lack of adequate genomic information. By sequencing cDNA libraries from the central nervous system (CNS), we have identified over 175,000 expressed sequence tags (ESTs), of which 19,814 are unique neuronal gene products and represent 50%-70% of the total Aplysia neuronal transcriptome. We have characterized the transcriptome at three levels: (1) the central nervous system, (2) the elementary components of a simple behavior: the gill-withdrawal reflex-by analyzing sensory, motor, and serotonergic modulatory neurons, and (3) processes of individual neurons. In addition to increasing the amount of available gene sequences of Aplysia by two orders of magnitude, this collection represents the largest database available for any member of the Lophotrochozoa and therefore provides additional insights into evolutionary strategies used by this highly successful diversified lineage, one of the three proposed superclades of bilateral animals.
AB - Molecular analyses of Aplysia, a well-established model organism for cellular and systems neural science, have been seriously handicapped by a lack of adequate genomic information. By sequencing cDNA libraries from the central nervous system (CNS), we have identified over 175,000 expressed sequence tags (ESTs), of which 19,814 are unique neuronal gene products and represent 50%-70% of the total Aplysia neuronal transcriptome. We have characterized the transcriptome at three levels: (1) the central nervous system, (2) the elementary components of a simple behavior: the gill-withdrawal reflex-by analyzing sensory, motor, and serotonergic modulatory neurons, and (3) processes of individual neurons. In addition to increasing the amount of available gene sequences of Aplysia by two orders of magnitude, this collection represents the largest database available for any member of the Lophotrochozoa and therefore provides additional insights into evolutionary strategies used by this highly successful diversified lineage, one of the three proposed superclades of bilateral animals.
UR - http://www.scopus.com/inward/record.url?scp=33845635268&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2006.09.052
DO - 10.1016/j.cell.2006.09.052
M3 - Article
C2 - 17190607
AN - SCOPUS:33845635268
SN - 0092-8674
VL - 127
SP - 1453
EP - 1467
JO - Cell
JF - Cell
IS - 7
ER -