Neurofibromatosis type 1 (Nf1)-mutant mice exhibit increased sleep fragmentation

Neurofibromatosis type 1 (NF1) is a neurodevelopmental disorder in which affected children and adults are at a higher risk of sleep disorders. In an effort to identify potential sleep disturbances in a small animal model, we used a previously reported Nf1 conditional knockout (Nf1^CKO) mouse strain. In contrast to Nf1 mutant flies, the distribution of vigilance states was intact in Nf1^CKO mice. However, Nf1^CKO mice exhibited increased non-REM sleep (NREM)-to-wake and wake-to-NREM transitions. This sleep disruption was accompanied by decreased bout durations during awake and NREM sleep states under both light and dark conditions. Moreover, Nf1^CKO mice have higher percentage delta power during awake and NREM sleep states under all light conditions. Taken together, Nf1^CKO mice phenocopy some of the sleep disturbances observed in NF1 patients and provide a tractable platform to explore the molecular mechanisms governing sleep abnormalities in NF1.