Neurofibromatosis type 1 gene product (neurofibromin) associates with microtubules

Paula E. Gregory, David H. Gutmann, Anna Mitchell, Soochul Park, Mark Boguski, Tyler Jacks, Deborah L. Wood, Richard Jove, Francis S. Collins

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

The neurofibromatosis type 1 (NF1) gene was recently identified by positional cloning and found to encode a protein with structural and functional homology to mammalian and yeast GTPase-activating proteins (GAPs). Using antibodies directed against the NF1 gene product, a protein of ∼250kDa was identified and termed neurofibromin. Double-indirect immunofluorescent labeling with anti-neurofibromin and anti-tubulin antibodies demonstrates that neurofibromin associates with cytoplasmic microtubules. Immunoblotting of microtubule-enriched cytoplasmic fractions, using antibodies generated against neurofibromin, shows that neurofibromin copurifies with microtubules. When portions of neurofibromin are expressed in Sf9 insect cells they associate with polymerized microtubules; furthermore, the critical residues for this interaction reside within the GAP-related domain of neurofibromin. The unexpected association of neurofibromin with microtubules suggests that neurofibromin is involved in microtubule-mediated intracellullar signal transduction pathways.

Original languageEnglish
Pages (from-to)265-274
Number of pages10
JournalSomatic Cell and Molecular Genetics
Volume19
Issue number3
DOIs
StatePublished - May 1993

Fingerprint Dive into the research topics of 'Neurofibromatosis type 1 gene product (neurofibromin) associates with microtubules'. Together they form a unique fingerprint.

Cite this