TY - JOUR
T1 - Neural progenitor genes
T2 - Germinal zone expression and analysis of genetic overlap in stem cell populations
AU - Easterday, Mathew C.
AU - Dougherty, Joseph D.
AU - Jackson, Robert L.
AU - Ou, Jing
AU - Nakano, Ichiro
AU - Paucar, Andres A.
AU - Roobini, Babak
AU - Dianati, Mehrnoosh
AU - Irvin, Dwain K.
AU - Weissman, Irving L.
AU - Terskikh, Alexey V.
AU - Geschwind, Daniel H.
AU - Kornblum, Harley I.
N1 - Funding Information:
This work was supported by NIMH grants MH065756 and MH60233. J.D.D. is supported by an HHMI predoctoral fellowship. R.L.J. is supported by supplement MH065756S from the NIMH. The authors thank Dr. Hong Wu for the embryonic stem cells.
PY - 2003/12/15
Y1 - 2003/12/15
N2 - The identification of the genes regulating neural progenitor cell (NPC) functions is of great importance to developmental neuroscience and neural repair. Previously, we combined genetic subtraction and microarray analysis to identify genes enriched in neural progenitor cultures. Here, we apply a strategy to further stratify the neural progenitor genes. In situ hybridization demonstrates expression in the central nervous system germinal zones of 54 clones so identified, making them highly relevant for study in brain and neural progenitor development. Using microarray analysis we find 73 genes enriched in three neural stem cell (NSC)-containing populations generated under different conditions. We use the custom microarray to identify 38 "stemness" genes, with enriched expression in the three NSC conditions and present in both embryonic stem cells and hematopoietic stem cells. However, comparison of expression profiles from these stem cell populations indicates that while there is shared gene expression, the amount of genetic overlap is no more than what would be expected by chance, indicating that different stem cells have largely different gene expression patterns. Taken together, these studies identify many genes not previously associated with neural progenitor cell biology and also provide a rational scheme for stratification of microarray data for functional analysis.
AB - The identification of the genes regulating neural progenitor cell (NPC) functions is of great importance to developmental neuroscience and neural repair. Previously, we combined genetic subtraction and microarray analysis to identify genes enriched in neural progenitor cultures. Here, we apply a strategy to further stratify the neural progenitor genes. In situ hybridization demonstrates expression in the central nervous system germinal zones of 54 clones so identified, making them highly relevant for study in brain and neural progenitor development. Using microarray analysis we find 73 genes enriched in three neural stem cell (NSC)-containing populations generated under different conditions. We use the custom microarray to identify 38 "stemness" genes, with enriched expression in the three NSC conditions and present in both embryonic stem cells and hematopoietic stem cells. However, comparison of expression profiles from these stem cell populations indicates that while there is shared gene expression, the amount of genetic overlap is no more than what would be expected by chance, indicating that different stem cells have largely different gene expression patterns. Taken together, these studies identify many genes not previously associated with neural progenitor cell biology and also provide a rational scheme for stratification of microarray data for functional analysis.
KW - Embryonic stem cell
KW - Germinal zone
KW - Hematopoietic stem cell
KW - Microarray
KW - Neural progenitor cell
KW - Neural stem cell
KW - Stemness
KW - Subventricular zone
KW - Ventricular zone
UR - http://www.scopus.com/inward/record.url?scp=0345357653&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2003.09.003
DO - 10.1016/j.ydbio.2003.09.003
M3 - Article
C2 - 14651920
AN - SCOPUS:0345357653
SN - 0012-1606
VL - 264
SP - 309
EP - 322
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -