Neural cell fate analysis in zebrafish using olig2 BAC transgenics

Jimann Shin, Hae Chul Park, Jolanta M. Topczewska, David J. Madwsley, Bruce Appel

Research output: Contribution to journalArticle

176 Scopus citations

Abstract

We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.

Original languageEnglish
Pages (from-to)7-14
Number of pages8
JournalMethods in Cell Science
Volume25
Issue number1-2
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

Keywords

  • Cerebellum
  • EGFP
  • Motor neuron
  • Olig2
  • Oligodendrocyte
  • Purkinje neuron
  • Spinal cord
  • Transgenic
  • Zebrafish

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