We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.
|Number of pages||8|
|Journal||Methods in Cell Science|
|State||Published - Dec 1 2003|
- Motor neuron
- Purkinje neuron
- Spinal cord