TY - JOUR
T1 - Nerve regeneration in silicone chambers
T2 - Influence of gap length and of distal stump components
AU - Lundborg, Göran
AU - Dahlin, Lars B.
AU - Danielsen, Nils
AU - Gelberman, Richard H.
AU - Longo, Frank M.
AU - Powell, Henry C.
AU - Varon, Silvio
N1 - Funding Information:
Council grant 5188, the Swedish Sciences, National Institutes of Veterans Administration, and a March of Dimes Foundation. Biology, M-001, University
Funding Information:
Abbreviations: PD-proximal-distal, PG-proximal-open. ’ This work was supported by Swedish Medical Research Work Environment Fund, the Swedish Society for Medical Health grants NS-16349, NS-14162, and AM-25501, the Basil O’Connor Starter Research Grant from the National Please send reprint requests to Dr. Varon, Department of California, San Diego, La Jolla, CA 92093.
PY - 1982/5
Y1 - 1982/5
N2 - The range of growth-promoting influences from a distal nerve stump on a regenerating proximal stump was determined using an experimental system in which a gap between cross-anastomosed rat sciatic nerves was encased by a cylindrical silicone chamber. Two arrangements were examined after 1 month in situ: A proximal-distal (PD) system in which both proximal and distal stumps were introduced into the ends of the chamber, and a proximal-open (PO) system in which the distal stump was omitted. When the gap was 6 mm long, a regenerated nerve extended all the way through the chamber in both the PD and PO systems. When the gap was increased to 10 mm, a similar regrowth occurred in the PD chamber, whereas in the PO chamber proximal regrowth was partial or nonexistent. When the gap was increased to 15 mm, no regeneration occurred, even in the presence of the distal stump. These observations confirm that the distal stump influences proximal regeneration and indicate that this influence can act only over a limited distance or volume. Such an influence could consist of humoral agents which support nerve growth and/or outgrowth from the distal stump.
AB - The range of growth-promoting influences from a distal nerve stump on a regenerating proximal stump was determined using an experimental system in which a gap between cross-anastomosed rat sciatic nerves was encased by a cylindrical silicone chamber. Two arrangements were examined after 1 month in situ: A proximal-distal (PD) system in which both proximal and distal stumps were introduced into the ends of the chamber, and a proximal-open (PO) system in which the distal stump was omitted. When the gap was 6 mm long, a regenerated nerve extended all the way through the chamber in both the PD and PO systems. When the gap was increased to 10 mm, a similar regrowth occurred in the PD chamber, whereas in the PO chamber proximal regrowth was partial or nonexistent. When the gap was increased to 15 mm, no regeneration occurred, even in the presence of the distal stump. These observations confirm that the distal stump influences proximal regeneration and indicate that this influence can act only over a limited distance or volume. Such an influence could consist of humoral agents which support nerve growth and/or outgrowth from the distal stump.
UR - http://www.scopus.com/inward/record.url?scp=0020033340&partnerID=8YFLogxK
U2 - 10.1016/0014-4886(82)90215-1
DO - 10.1016/0014-4886(82)90215-1
M3 - Article
C2 - 7095058
AN - SCOPUS:0020033340
SN - 0014-4886
VL - 76
SP - 361
EP - 375
JO - Experimental Neurology
JF - Experimental Neurology
IS - 2
ER -