TY - JOUR
T1 - Nerve crush injuries-a model for axonotmesis
AU - Bridge, Peter M.
AU - Ball, Douglas J.
AU - Mackinnon, Susan E.
AU - Nakao, Yasushi
AU - Brandt, Keith
AU - Hunter, Daniel A.
AU - Hertl, Catherine
PY - 1994/6
Y1 - 1994/6
N2 - Nerve crush is a commonly used experimental model in the rat; however, a standard method of inducing this injury has not been defined. This study examined six crush techniques that are frequently used and characterized the subsequent nerve injury. Five types of nerve crush using a No. 5 jeweler’s forceps and a sixth using a 30-s single crush with a serrated hemostat were studied in the posterior tibial nerve of the Lewis rat. Regeneration was evaluated using serial walking track assessments at 1, 2, 4, 6, and 8 weeks postoperatively. Nerve conduction studies and histological examination were performed at 2 days, 2 weeks, and 8 weeks. Blood-nerve barrier breakdown was observed at 2 days and recovered by 2 weeks. By 4 weeks normal walking track patterns were obtained in all groups. A pattern of Wallerian degeneration and axonal regeneration was noted at 2 weeks, with histological recovery in all groups by 8 weeks. Nerve crush, induced by any of the six methods tested, was similar and provides a reliable model of axonotmesis.
AB - Nerve crush is a commonly used experimental model in the rat; however, a standard method of inducing this injury has not been defined. This study examined six crush techniques that are frequently used and characterized the subsequent nerve injury. Five types of nerve crush using a No. 5 jeweler’s forceps and a sixth using a 30-s single crush with a serrated hemostat were studied in the posterior tibial nerve of the Lewis rat. Regeneration was evaluated using serial walking track assessments at 1, 2, 4, 6, and 8 weeks postoperatively. Nerve conduction studies and histological examination were performed at 2 days, 2 weeks, and 8 weeks. Blood-nerve barrier breakdown was observed at 2 days and recovered by 2 weeks. By 4 weeks normal walking track patterns were obtained in all groups. A pattern of Wallerian degeneration and axonal regeneration was noted at 2 weeks, with histological recovery in all groups by 8 weeks. Nerve crush, induced by any of the six methods tested, was similar and provides a reliable model of axonotmesis.
UR - http://www.scopus.com/inward/record.url?scp=0028067228&partnerID=8YFLogxK
U2 - 10.1006/exnr.1994.1104
DO - 10.1006/exnr.1994.1104
M3 - Article
C2 - 8033968
AN - SCOPUS:0028067228
SN - 0014-4886
VL - 127
SP - 284
EP - 290
JO - Experimental Neurology
JF - Experimental Neurology
IS - 2
ER -