Neonatal necrotizing enterocolitis-associated DNA methylation signatures in the colon are evident in stool samples of affected individuals

Misty Good, Tianjiao Chu, Patricia Shaw, Lila S. Nolan, Lora McClain, Austin Chamberlain, Carlos Castro, Qingqing Gong, Krista Cooksey, Laura Linneman, Olivia N. Dewitt, David N. Finegold, David G. Peters

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: Neonatal necrotizing enterocolitis (NEC) is a deadly and unpredictable gastrointestinal disease, for which no biomarker exists. We aimed to describe the methylation patterns in stool and colon from infants with NEC. Methods: We performed a high-resolution genome-wide epigenomic analysis using solution-phase hybridization and next-generation sequencing of bisulfite-converted DNA. Results: Our data reveal significant genomic hypermethylation in NEC tissues compared with non-NEC controls. These changes were more pronounced in regions outside CpG islands and gene regulatory elements, suggesting that NEC-specific hypermethylation is not a nonspecific global phenomenon. Conclusions: This study provides evidence of a methylomic signature associated with NEC that is detectable noninvasively and provides a new opportunity for the development of a novel diagnostic method for NEC.

Original languageEnglish
Pages (from-to)829-844
Number of pages16
JournalEpigenomics
Volume13
Issue number11
DOIs
StatePublished - Jun 2021

Keywords

  • biomarker
  • colon
  • DNA methylation
  • epigenetics
  • intestine
  • methylome
  • necrotizing enterocolitis
  • neonatal
  • prematurity
  • stool

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