TY - JOUR
T1 - Near-infrared angiography for critical limb ischemia in a diabetic murine model
AU - Garcia, Missael
AU - Zayed, Mohamed A.
AU - Park, Kyoung Mi
AU - Gruev, Viktor
N1 - Publisher Copyright:
© 2017 Society of Photo-Optical Instrumentation Engineers (SPIE).
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Peripheral arterial disease (PAD) is a highly prevalent disease process that afflicts more than 20% of individuals with diabetes. Progression of PAD in the setting of diabetes can lead to critical limb ischemia (CLI), which is associated with increased risk of wounds, gangrene, and limb loss. Prompt noninvasive evaluation of limbs affected by PAD progression and CLI is currently limited. Here, we evaluate the utility of a custom-designed multispectral imaging system for fluorescence-based near-infrared angiography and compare it to the existing gold standard of laser-scanning Doppler perfusion assessments. Due to its higher resolution and fluorescence sensitivity, near-infrared angiography demonstrates a greater capacity to characterize altered dynamic arterial perfusion in a clinically relevant diabetic murine model for CLI. Furthermore, we demonstrate that our imaging system can accurately track arterial perfusion recovery over time following induced ischemia, and reveal unique phenotypic differences in the setting of diabetes.
AB - Peripheral arterial disease (PAD) is a highly prevalent disease process that afflicts more than 20% of individuals with diabetes. Progression of PAD in the setting of diabetes can lead to critical limb ischemia (CLI), which is associated with increased risk of wounds, gangrene, and limb loss. Prompt noninvasive evaluation of limbs affected by PAD progression and CLI is currently limited. Here, we evaluate the utility of a custom-designed multispectral imaging system for fluorescence-based near-infrared angiography and compare it to the existing gold standard of laser-scanning Doppler perfusion assessments. Due to its higher resolution and fluorescence sensitivity, near-infrared angiography demonstrates a greater capacity to characterize altered dynamic arterial perfusion in a clinically relevant diabetic murine model for CLI. Furthermore, we demonstrate that our imaging system can accurately track arterial perfusion recovery over time following induced ischemia, and reveal unique phenotypic differences in the setting of diabetes.
KW - critical limb ischemia
KW - diabetes
KW - indocyanine green
KW - near-infrared angiography
UR - http://www.scopus.com/inward/record.url?scp=85021768723&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.22.4.046006
DO - 10.1117/1.JBO.22.4.046006
M3 - Article
C2 - 28418536
AN - SCOPUS:85021768723
SN - 1083-3668
VL - 22
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 4
M1 - 046006
ER -