Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells

Sujan Shresta, Debra M. Macivor, Jonathan W. Heusel, John H. Russell, Timothy J. Ley

Research output: Contribution to journalArticlepeer-review

201 Scopus citations

Abstract

Granzyme (Gzm) B-deficient mice obtained by gene targeting were used to assess the role of Gzm B in the mechanisms used by natural killer (NK) and lymphokine-activated killer (LAK) cells to destroy target cells. Gzm B(-/-) NK cells, LAK cells, and cytotoxic T lymphocytes (CTL) all are defective in their ability to rapidly induce DNA fragmentation/apoptosis in susceptible target cells. This defect can be partially corrected with long incubation times of effector and target cells. Moreover, Gzm B(-/-) NK cells (but not CTL or LAK cells) exhibit a defect in 51Cr release from susceptible target cells. This 51Cr release defect in Gzm B-deficient NK cells is also not overcome by prolonged incubation times or high effector-to-target cell ratios. We conclude that Gzm B plays a critical and nonredundant role in the rapid induction of DNA fragmentation/apoptosis by NK cells, LAK cells, and CTL. Gzm B may have an additional role in NK cells (but not in CTL or LAK cells) for mediating 51Cr release.

Original languageEnglish
Pages (from-to)5679-5683
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number12
DOIs
StatePublished - Jun 6 1995

Keywords

  • cell-mediated cytotoxicity

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