Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels

Yang Pu, Jianpeng Xue, Wubao Wang, Baogang Xu, Yueqing Gu, Rui Tang, Ellen Ackerstaff, Jason A. Koutcher, Samuel Achilefu, Robert R. Alfano

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.

Original languageEnglish
Article number087002
JournalJournal of biomedical optics
Volume18
Issue number8
DOIs
StatePublished - Aug 1 2013

Keywords

  • NADH
  • metastasisrelevant key fluorophores
  • moderately metastatic and advanced metastatic cell lines
  • native fluorescence spectra
  • normal
  • optical biopsy
  • tryptophan

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