Methicillin-resistente Staphylococcus aureus (MRSA) im nasenabstrich von Beschäftigten im schweizer veterinärsektor: Nachweis von nutztier-assoziierten und spital-assoziierten klonen

Translated title of the contribution: Nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among Swiss veterinary health care providers: Detection of livestock- and healthcare-associated clones

K. Wettstein Rosenkranz, E. Rothenanger, I. Brodard, A. Collaud, G. Overesch, B. Bigler, J. Marschall, V. Perreten

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We screened a total of 340 veterinarians (including general practitioners, small animal practitioners, large animal practitioners, veterinarians working in different veterinary services or industry), and 29 veterinary assistants for nasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus pseudintermedius (MRSP) at the 2012 Swiss veterinary annual meeting. MRSA isolates (n = 14) were detected in 3.8 % (95 % CI 2.1-6.3 %) of the participants whereas MRSP was not detected. Large animal practitioners were carriers of livestock-associated MRSA (LA-MRSA) ST398-t011-V (n = 2), ST398-t011-IV (n = 4), and ST398-t034-V (n = 1). On the other hand, participants working with small animals harbored human healthcare-associated MRSA (HCA-MRSA) which belonged to epidemic lineages ST225-t003-II (n = 2), ST225-t014-II (n = 1), ST5-t002-II (n = 2), ST5-t283-IV (n = 1), and ST88-t186-IV (n = 1). HCAMRSA harbored virulence factors such as enterotoxins, β-hemolysin converting phage and leukocidins. None of the MRSA isolates carried Panton-Valentine leukocidin (PVL). In addition to the methicillin resistance gene mecA, LA-MRSA ST398 isolates generally contained additional antibiotic resistance genes conferring resistance to tetracycline [tet(M) and tet(K)], trimethoprim [dfrK, dfrG], and the aminoglycosides gentamicin and kanamycin [aac(6')-Ie-aph(2')- Ia]. On the other hand, HCA-MRSA ST5 and ST225 mainly contained genes conferring resistance to the macrolide, lincosamide and streptogramin B antibiotics [erm(A)], to spectinomycin [ant(9)-Ia], amikacin and tobramycin [ant(4')-Ia], and to fl uoroquinolones [amino acid substitutions in GrlA (S84L) and GyrA (S80F and S81P)]. MRSA carriage may represent an occupational risk and veterinarians should be aware of possible MRSA colonization and potential for developing infection or for transmitting these strains. Professional exposure to animals should be reported upon hospitalization and before medical intervention to allow for preventive measures. Infection prevention measures are also indicated in veterinary medicine to avoid MRSA transmission between humans and animals, and to limit the spread of MRSA both in the community, and to animal and human hospitals.

Translated title of the contributionNasal carriage of methicillin-resistant Staphylococcus aureus (MRSA) among Swiss veterinary health care providers: Detection of livestock- and healthcare-associated clones
Original languageGerman
Pages (from-to)317-325
Number of pages9
JournalSchweizer Archiv fur Tierheilkunde
Volume156
Issue number7
DOIs
StatePublished - Jul 2014

Keywords

  • Antibiotic resistance
  • Genotyping
  • Human
  • Meca
  • Nose
  • Prevalence
  • S. pseudintermedius
  • Staphylococcus aureus

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