Nardilysin convertase regulates the function of the maxi-K channel isoform mK44 in human myometrium

Victoria P. Korovkina, Susan J. Stamnes, Adam M. Brainard, Sarah K. England

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In smooth muscle, large-conductance Ca2+- and voltage-activated K+ channels from the gene KCNMA (maxi-K channels) generate isoforms with disparate responses to contractile stimuli. We previously showed that the human myometrium expresses high levels of the splice variant of the maxi-K channel containing a 44-amino acid insertion (mK44). The studies presented here demonstrate that nardilysin convertase, a Zn2+-dependent metalloprotease of the insulinase family, regulates the plasma membrane expression of mK44 and its response to increases in intracellular Ca 2+. We show that nardilysin convertase isoform 1 is present in human myometrium and colocalizes with mK44. Studies indicate that nardilysin convertase regulates 1) retention of the mK44 COOH-terminal fragment in the endoplasmic reticulum in quiescent myometrial smooth muscle and 2) Ca 2+-induced translocation of mK44 to the plasma membrane. In mouse fibroblasts, nardilysin convertase significantly attenuates mK44-dependent current. In human myometrial smooth muscle cells, inhibition of nardilysin convertase promotes membrane localization of mK44 and an increase in maxi-K current. Overall, our data indicate that, in human myometrium, nardilysin convertase and mK44 channels are a part of the molecular mechanism that regulates the excitability of smooth muscle cells.

Original languageEnglish
Pages (from-to)C433-C440
JournalAmerican Journal of Physiology - Cell Physiology
Volume296
Issue number3
DOIs
StatePublished - Mar 2009

Keywords

  • Large-conductance calcium-activated potassium channel
  • N-arginine dibasic convertase
  • Smooth muscle

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