TY - JOUR
T1 - Nanostructure initiator mass spectrometry
T2 - Tissue imaging and direct biofluid analysis
AU - Yanes, Oscar
AU - Woo, Hin Koon
AU - Northen, Trent R.
AU - Oppenheimer, Stacey R.
AU - Shriver, Leah
AU - Apon, Jon
AU - Estrada, Mayra N.
AU - Potchoiba, Michael J.
AU - Steenwyk, Rick
AU - Manchester, Marianne
AU - Siuzdak, Gary
PY - 2009/4/15
Y1 - 2009/4/15
N2 - Nanostructure initiator mass spectrometry (NIMS) is a recently introduced matrix-free desorption/ionization platform that requires minimal sample preparation. Its application to xenobiotics and endogenous metabolites in tissues is demonstrated, where clozapine and N-desm-ethylclozapine were observed from mouse and rat brain sections. It has also been applied to direct biofluid analysis where ketamine and norketamine were observed from plasma and urine. Detection of xenobiotics from biofluids was made even more effective using a novel NIMS on-surface extraction method taking advantage of the hydrophobic nature of the initiator. Linear response and limit of detection were also evaluated for xenobiotics such as methamphetamine, codeine, alprazolam, and morphine, revealing that NIMS can be used for quantitative analysis. Overall, our results demonstrate the capacity of NIMS to perform sensitive, simple, and rapid analyses from highly complex biological tissues and fluids.
AB - Nanostructure initiator mass spectrometry (NIMS) is a recently introduced matrix-free desorption/ionization platform that requires minimal sample preparation. Its application to xenobiotics and endogenous metabolites in tissues is demonstrated, where clozapine and N-desm-ethylclozapine were observed from mouse and rat brain sections. It has also been applied to direct biofluid analysis where ketamine and norketamine were observed from plasma and urine. Detection of xenobiotics from biofluids was made even more effective using a novel NIMS on-surface extraction method taking advantage of the hydrophobic nature of the initiator. Linear response and limit of detection were also evaluated for xenobiotics such as methamphetamine, codeine, alprazolam, and morphine, revealing that NIMS can be used for quantitative analysis. Overall, our results demonstrate the capacity of NIMS to perform sensitive, simple, and rapid analyses from highly complex biological tissues and fluids.
UR - https://www.scopus.com/pages/publications/65249165397
U2 - 10.1021/ac802576q
DO - 10.1021/ac802576q
M3 - Article
C2 - 19301920
AN - SCOPUS:65249165397
SN - 0003-2700
VL - 81
SP - 2969
EP - 2975
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 8
ER -