Abstract
Strategies to enhance survival and direct the differentiation of stem cells in vivo following transplantation in tissue repair site are critical to realizing the potential of stem cell-based therapies. Here we demonstrated an effective approach to promote neuronal differentiation and maturation of human fetal tissue-derived neural stem cells (hNSCs) in a brain lesion site of a rat traumatic brain injury model using biodegradable nanoparticle-mediated transfection method to deliver key transcriptional factor neurogenin-2 to hNSCs when transplanted with a tailored hyaluronic acid (HA) hydrogel, generating larger number of more mature neurons engrafted to the host brain tissue than non-transfected cells. The nanoparticle-mediated transcription activation method together with an HA hydrogel delivery matrix provides a translatable approach for stem cell-based regenerative therapy.
Original language | English |
---|---|
Pages (from-to) | 157-166 |
Number of pages | 10 |
Journal | Biomaterials |
Volume | 84 |
DOIs | |
State | Published - Apr 1 2016 |
Keywords
- Nanoparticles
- Neurogenin-2
- Neuronal differentiation
- Transfection
- Traumatic brain injury