Nano-PCR - Vývoj novej metódy pre detekciu extrémne nízkych koncentracií bielkovín hrajúcich úlohu v patologických procesoch

Filip Franko; M. Bambousková; L. Potůčková; P. Dráber

Nano-PCR - Vývoj novej metódy pre detekciu extrémne nízkych koncentracií bielkovín hrajúcich úlohu v patologických procesoch

Translated title of the contribution: Nano-PCR - Development of a new method for detection extremely low levels of proteins important in pathological processes

Filip Franko, M. Bambousková, L. Potůčková, P. Dráber

Research output: Contribution to journal › Article › peer-review

Abstract

We are developing a novel approach based on ELISA using gold nanoparticles which allows determination of appropriate markers in the blood serum whereby solving several problems in biochemical diagnostic of neurodegenerative processes. Signal amplification is achieved by binding of selected antibody to gold nanoparticle covered with DNA fragments, followed by DNA amplification and real-time PCR determination. This approach provides higher senstitivity compared to the recently used clinical methods.

Translated title of the contribution	Nano-PCR - Development of a new method for detection extremely low levels of proteins important in pathological processes
Original language	Slovak
Pages (from-to)	29-30
Number of pages	2
Journal	Psychiatrie
Volume	13
Issue number	SUPPL. 2
State	Published - 2009

Keywords

Alzheimer's disease
Gold nanoparticles
Immuno-PCR
Real-time PCR

Cite this

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abstract = "We are developing a novel approach based on ELISA using gold nanoparticles which allows determination of appropriate markers in the blood serum whereby solving several problems in biochemical diagnostic of neurodegenerative processes. Signal amplification is achieved by binding of selected antibody to gold nanoparticle covered with DNA fragments, followed by DNA amplification and real-time PCR determination. This approach provides higher senstitivity compared to the recently used clinical methods.",

keywords = "Alzheimer's disease, Gold nanoparticles, Immuno-PCR, Real-time PCR",

author = "Filip Franko and M. Bambouskov{\'a} and L. Potů{\v c}kov{\'a} and P. Dr{\'a}ber",

year = "2009",

language = "Slovak",

volume = "13",

pages = "29--30",

journal = "Psychiatrie",

issn = "1211-7579",

number = "SUPPL. 2",

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T1 - Nano-PCR - Vývoj novej metódy pre detekciu extrémne nízkych koncentracií bielkovín hrajúcich úlohu v patologických procesoch

AU - Franko, Filip

AU - Bambousková, M.

AU - Potůčková, L.

AU - Dráber, P.

PY - 2009

Y1 - 2009

N2 - We are developing a novel approach based on ELISA using gold nanoparticles which allows determination of appropriate markers in the blood serum whereby solving several problems in biochemical diagnostic of neurodegenerative processes. Signal amplification is achieved by binding of selected antibody to gold nanoparticle covered with DNA fragments, followed by DNA amplification and real-time PCR determination. This approach provides higher senstitivity compared to the recently used clinical methods.

AB - We are developing a novel approach based on ELISA using gold nanoparticles which allows determination of appropriate markers in the blood serum whereby solving several problems in biochemical diagnostic of neurodegenerative processes. Signal amplification is achieved by binding of selected antibody to gold nanoparticle covered with DNA fragments, followed by DNA amplification and real-time PCR determination. This approach provides higher senstitivity compared to the recently used clinical methods.

KW - Alzheimer's disease

KW - Gold nanoparticles

KW - Immuno-PCR

KW - Real-time PCR

UR - http://www.scopus.com/inward/record.url?scp=67749099770&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:67749099770

SN - 1211-7579

VL - 13

SP - 29

EP - 30

JO - Psychiatrie

JF - Psychiatrie

IS - SUPPL. 2

ER -

Nano-PCR - Vývoj novej metódy pre detekciu extrémne nízkych koncentracií bielkovín hrajúcich úlohu v patologických procesoch

Abstract

Keywords

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