N-terminal deletions and His-tag fusions dramatically affect expression of cytochrome P450 2C2 in bacteria

Keyphrases

• His-tag 100%
• Cytochrome P450 (CYP450) 100%
• P450 100%
• Affect Expression 100%
• N-terminal Deletion 100%
• Green Fluorescent Protein 40%
• Transmembrane Domain 40%
• Linker Sequence 40%
• Signal Anchor 40%
• Reduced Expression 20%
• Translocated 20%
• Membrane Targeting 20%
• Hydrophobic Interaction 20%
• Escherichia Coli 20%
• High-alkaline 20%
• High Salt 20%
• Linker 20%
• Integral Membrane Protein 20%
• N-terminus 20%
• Expression Level 20%
• Fusion Protein 20%
• Fluorescence Microscopy 20%
• Insect Cells 20%
• Membrane-associated 20%
• N-terminal Sequence 20%
• Multifunctional Protein 20%
• Subcellular Fractionation 20%
• N-terminal Transmembrane Domain 20%
• Bacterial Growth 20%
• Peripheral Membrane Protein 20%
• Cytosol 20%

Biochemistry, Genetics and Molecular Biology

• N-Terminus 100%
• Cytochrome P450 100%
• Bacterium 100%
• Transmembrane Domain 37%
• Green Fluorescent Protein 25%
• Wild Type 12%
• Escherichia coli 12%
• Fluorescence Microscopy 12%
• Deletion Mutant 12%
• Cell Fractionation 12%
• Integral Membrane Protein 12%
• Bacterial Growth 12%
• Peripheral Membrane Protein 12%
• Chimeric Protein 12%
• Expression Level 12%

Immunology and Microbiology

• Amino Terminal Sequence 100%
• Cytochrome P450 100%
• Bacterium 100%
• Wild Type 16%
• Cell Fractionation 16%
• Cytosol 16%
• Escherichia coli 16%
• Deletion Mutant 16%
• Insect Cell 16%
• Bacterial Growth 16%
• Fluorescence Microscopy 16%