N-Methyl-D-asparate Receptor Agonists Decrease Protooncogene bcl-2 mRNA Expression in Cultured Rat Cerebellar Granule Neurons

P. Montpied, M. Weller, S. M. Paul

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The protooncogene bcl-2 was studied as a candidate gene for N-methyl-D-aspartate(NMDA)-induced excitoprotection of cerebellar granule neurons. Exposure of these neurons to excitoprotective concentrations of NMDA and to excitotoxic concentrations of glutamate decreased bcl-2 mRNA levels. Preexposure to NMDA failed to modify the glutamate-evoked decrease in bcl-2 mRNA but increased neuronal survival from 20% to 100%. Neither the intracellular mechanisms underlying the cascade of excitoprotection induced by NMDA nor those underlying the cascade of glutamate toxicity of cerebellar granule neurons seem to specifically involve changes in bcl-2 mRNA levels. Glutamate did not induce nucleosomal DNA fragmentation but glutamate toxicity was inhibited by aurintricarboxylic acid. Glutamate toxicity of cerebellar granule neurons differs considerably from classical programmed cell death.

Original languageEnglish
Pages (from-to)623-629
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume195
Issue number2
DOIs
StatePublished - Sep 15 1993
Externally publishedYes

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