N-linked glycosylation of dengue virus NS1 protein modulates secretion, cell-surface expression, hexamer stability, and interactions with human complement

Pawit Somnuke, Richard E. Hauhart, John P. Atkinson, Michael S. Diamond, Panisadee Avirutnan

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

Dengue virus (DENV) NS1 is a versatile non-structural glycoprotein that is secreted as a hexamer, binds to the cell surface of infected and uninfected cells, and has immune evasive functions. DENV NS1 displays two conserved N-linked glycans at N130 and N207. In this study, we examined the role of these two N-linked glycans on NS1 secretion, stability, and function. Because some groups have reported reduced yields of infectious DENV when N130 and N207 are changed, we analyzed glycosylation-deficient NS1 phenotypes using a transgenic expression system. We show that the N-linked glycan at position 130 is required for stabilization of the secreted hexamer whereas the N-linked glycan at residue 207 facilitates secretion and extracellular protein stability. Moreover, NS1 mutants lacking an N-linked glycan at N130 did not interact efficiently with complement components C1s and C4. In summary, our results elucidate the contribution of N-linked glycosylation to the function of DENV NS1.

Original languageEnglish
Pages (from-to)253-264
Number of pages12
JournalVirology
Volume413
Issue number2
DOIs
StatePublished - May 10 2011

Keywords

  • Complement
  • Dengue virus
  • Flavivirus
  • N-linked glycosylation
  • Non-structural protein nS1

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