Myristoylation-Enhanced Binding of the HIV-1 Net Protein to T Cell Skeletal Matrix

Thomas M.J. Niederman, W. Randall Hastings, Lee Ratner

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

The negative factor, Nef, of HIV-1 was found to associate to an extent of 16-42% with the detergent insoluble cytoskeletal fraction of T lymphocytes. Furthermore, Escherichia coli expressed Nef protein was found to bind during in vitro reactions with the cytoskeletal matrix to an extent of 30-50%. Cytoskeletal association of Nef was significantly enhanced by myristoylation. The specificity of the myristoylation-enhanced binding was demonstrated by the lack of an effect of myristoylation on binding of the HIV-1 Gag protein to the cytoskeleton. Cytoskeletal binding was saturable, and inhibited by high concentrations of sodium chloride, or with SDS or urea. Binding of Nef to the cytoskeletal matrix may be important in mediating its effects on HIV-1 replication.

Original languageEnglish
Pages (from-to)420-425
Number of pages6
JournalVirology
Volume197
Issue number1
DOIs
StatePublished - Nov 1993

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