@article{86ce2c68213f4ed5abbd5a865ab32f3f,
title = "Myostatin is a negative regulator of adult neurogenesis after spinal cord injury in zebrafish",
abstract = "Intrinsic and extrinsic inhibition of neuronal regeneration obstruct spinal cord (SC) repair in mammals. In contrast, adult zebrafish achieve functional recovery after complete SC transection. While studies of innate SC regeneration have focused on axon regrowth as a primary repair mechanism, how local adult neurogenesis affects functional recovery is unknown. Here, we uncover dynamic expression of zebrafish myostatin b (mstnb) in a niche of dorsal SC progenitors after injury. mstnb mutants show impaired functional recovery, normal glial and axonal bridging across the lesion, and an increase in the profiles of newborn neurons. Molecularly, neuron differentiation genes are upregulated, while the neural stem cell maintenance gene fgf1b is downregulated in mstnb mutants. Finally, we show that human fibroblast growth factor 1 (FGF1) treatment rescues the molecular and cellular phenotypes of mstnb mutants. These studies uncover unanticipated neurogenic functions for mstnb and establish the importance of local adult neurogenesis for innate SC repair.",
keywords = "CP: Developmental biology, CP: Neuroscience, adult neurogenesis, eural stem cells, myostatin, neuronal differentiation, regeneration, spinal cord injury, zebrafish",
author = "Saraswathy, {Vishnu Muraleedharan} and Lili Zhou and McAdow, {Anthony R.} and Brooke Burris and Deepika Dogra and Sven Reischauer and Mokalled, {Mayssa H.}",
note = "Funding Information: We thank V. Cavalli, A. Johnson, K. Poss, and L. Solnica-Krezel for discussions; D. Ornitz and D. Stainier for sharing lines and reagents; S. Higashijima, the National Institutes of Natural Sciences , and the National Bioresource Project of Japan as the source of the dbx1b:GFP line; T. Li and B. Zhang for bioinformatics analysis; and the Washington University Zebrafish Shared Resource for animal care. This research was supported by grants from the NIH ( R01 NS113915 to M.H.M.) and the McDonnell Center for Cellular Neuroscience (to M.H.M.). Funding Information: We thank V. Cavalli, A. Johnson, K. Poss, and L. Solnica-Krezel for discussions; D. Ornitz and D. Stainier for sharing lines and reagents; S. Higashijima, the National Institutes of Natural Sciences, and the National Bioresource Project of Japan as the source of the dbx1b:GFP line; T. Li and B. Zhang for bioinformatics analysis; and the Washington University Zebrafish Shared Resource for animal care. This research was supported by grants from the NIH (R01 NS113915 to M.H.M.) and the McDonnell Center for Cellular Neuroscience (to M.H.M.). V.M.S. L.Z. and M.H.M. designed the study, conducted experiments, and prepared the manuscript. A.R.M. and B.B. performed experiments. D.D. and S.R. generated mstnb mutants. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = nov,
day = "22",
doi = "10.1016/j.celrep.2022.111705",
language = "English",
volume = "41",
journal = "Cell Reports",
issn = "2211-1247",
number = "8",
}