Myometrial maxi-K channel β1 subunit modulation during pregnancy and after 17β-estradiol stimulation

Nancy A. Benkusky; Victoria P. Korovkina; Adam M. Brainard; Sarah K. England

doi:10.1016/S0014-5793(02)03011-9

Myometrial maxi-K channel β1 subunit modulation during pregnancy and after 17β-estradiol stimulation

Nancy A. Benkusky, Victoria P. Korovkina, Adam M. Brainard, Sarah K. England

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Myometrial maxi-K channels are modulated by β subunits. We aimed to determine whether β subunits are modulated to affect uterine excitability during gestation. RNase protection analyses revealed that mouse β1 subunit transcripts are regulated during gestation with peak expression at day 14 of pregnancy. Immunohistochemical analysis indicates an increase of this subunit during gestation. Upregulation of the β1 transcript occurs with 4-day exposure to 17β-estradiol but not progesterone, and acute estradiol exposure has no effect on β1 transcript expression. These findings verify that β1 subunit transcript is regulated in mouse myometrium during gestation and estrogens may contribute to this increase.

Original language	English
Pages (from-to)	97-102
Number of pages	6
Journal	FEBS Letters
Volume	524
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(02)03011-9
State	Published - Jul 31 2002

Keywords

Estrogen
Gestation
Maxi-K
Mouse
Progesterone
Uterus
β Subunit

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10.1016/S0014-5793(02)03011-9

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title = "Myometrial maxi-K channel β1 subunit modulation during pregnancy and after 17β-estradiol stimulation",

abstract = "Myometrial maxi-K channels are modulated by β subunits. We aimed to determine whether β subunits are modulated to affect uterine excitability during gestation. RNase protection analyses revealed that mouse β1 subunit transcripts are regulated during gestation with peak expression at day 14 of pregnancy. Immunohistochemical analysis indicates an increase of this subunit during gestation. Upregulation of the β1 transcript occurs with 4-day exposure to 17β-estradiol but not progesterone, and acute estradiol exposure has no effect on β1 transcript expression. These findings verify that β1 subunit transcript is regulated in mouse myometrium during gestation and estrogens may contribute to this increase.",

keywords = "Estrogen, Gestation, Maxi-K, Mouse, Progesterone, Uterus, β Subunit",

author = "Benkusky, {Nancy A.} and Korovkina, {Victoria P.} and Brainard, {Adam M.} and England, {Sarah K.}",

note = "Funding Information: The authors thank Mr. Daniel Fergus for critical review of the manuscript. This work was supported by National Institutes of Health Grants HD-37831 and HD-01371 (to S.K.E.) and American Heart Association Postdoctoral Fellowship 0120580Z (to V.P.K.). Reagents for these studies were provided by the Diabetes and Endocrinology Research Center (DK-25295) and the Gene Transfer and Vector Core (DK-54759) at the University of Iowa. ",

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AU - Benkusky, Nancy A.

AU - Korovkina, Victoria P.

AU - Brainard, Adam M.

AU - England, Sarah K.

N1 - Funding Information: The authors thank Mr. Daniel Fergus for critical review of the manuscript. This work was supported by National Institutes of Health Grants HD-37831 and HD-01371 (to S.K.E.) and American Heart Association Postdoctoral Fellowship 0120580Z (to V.P.K.). Reagents for these studies were provided by the Diabetes and Endocrinology Research Center (DK-25295) and the Gene Transfer and Vector Core (DK-54759) at the University of Iowa.

PY - 2002/7/31

Y1 - 2002/7/31

N2 - Myometrial maxi-K channels are modulated by β subunits. We aimed to determine whether β subunits are modulated to affect uterine excitability during gestation. RNase protection analyses revealed that mouse β1 subunit transcripts are regulated during gestation with peak expression at day 14 of pregnancy. Immunohistochemical analysis indicates an increase of this subunit during gestation. Upregulation of the β1 transcript occurs with 4-day exposure to 17β-estradiol but not progesterone, and acute estradiol exposure has no effect on β1 transcript expression. These findings verify that β1 subunit transcript is regulated in mouse myometrium during gestation and estrogens may contribute to this increase.

AB - Myometrial maxi-K channels are modulated by β subunits. We aimed to determine whether β subunits are modulated to affect uterine excitability during gestation. RNase protection analyses revealed that mouse β1 subunit transcripts are regulated during gestation with peak expression at day 14 of pregnancy. Immunohistochemical analysis indicates an increase of this subunit during gestation. Upregulation of the β1 transcript occurs with 4-day exposure to 17β-estradiol but not progesterone, and acute estradiol exposure has no effect on β1 transcript expression. These findings verify that β1 subunit transcript is regulated in mouse myometrium during gestation and estrogens may contribute to this increase.

KW - Estrogen

KW - Gestation

KW - Maxi-K

KW - Mouse

KW - Progesterone

KW - Uterus

KW - β Subunit

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DO - 10.1016/S0014-5793(02)03011-9

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EP - 102

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Myometrial maxi-K channel β1 subunit modulation during pregnancy and after 17β-estradiol stimulation

Abstract

Keywords

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