TY - JOUR
T1 - Myocardial protection with pinacidil cardioplegia in the blood-perfused heart
AU - Lawton, Jennifer S.
AU - Harrington, Gary C.
AU - Allen, Cynthia T.
AU - Hsia, Peng Wie
AU - Damiano, Ralph J.
PY - 1996/6
Y1 - 1996/6
N2 - Background. Adenosine triphosphate-sensitive potassium-channel openers are potent vasodilators that have been found to be cardioprotective during myocardial ischemia. The potassium-channel opener pinacidil was investigated to determine its efficacy as a cardioplegic agent. Methods. A blood-perfused, parabiotic, isolated rabbit heart Langendorff preparation was used. Fifty- six hearts underwent 30 minutes of global normothermic ischemia after a 50- mL infusion of cardioplegia, followed by 60 minutes of reperfusion. The cardioplegia consisted of Krebs-Henseleit solution with either vehicle alone (control), 20 mmol KCl, or pinacidil (10, 50, 100, 150, or 200 μmol/L). The developed pressure was measured at baseline and after reperfusion. Coronary blood flow was measured with an in-line ultrasonic probe. Results. Pinacidil (50 μmol/L), as opposed to potassium cardioplegia, provided significantly better postischemic percentage recovery of developed pressure compared with controls (68.3% ± 4.0%, versus 44.6% ± 5.5%; p < 0.05). The time until electrical arrest was significantly shorter in the hyperkalemic group than in all other groups. Linear end-diastolic pressure-volume relationships revealed an increase in slope after ischemia in all groups. Coronary flow after 5 minutes of reperfusion was significantly higher in both the 50-μmol/L and 100-μmol/L pinacidil groups compared with traditional hyperkalemic arrest, and this returned to baseline after 15 minutes. Conclusions. The potassium channel opener pinacidil provided dose-dependent myocardial protection during global ischemia in the blood-perfused rabbit heart model. Potassium-channel openers are a promising class of drugs that may provide an alternative to traditional hyperkalemic cardioplegia.
AB - Background. Adenosine triphosphate-sensitive potassium-channel openers are potent vasodilators that have been found to be cardioprotective during myocardial ischemia. The potassium-channel opener pinacidil was investigated to determine its efficacy as a cardioplegic agent. Methods. A blood-perfused, parabiotic, isolated rabbit heart Langendorff preparation was used. Fifty- six hearts underwent 30 minutes of global normothermic ischemia after a 50- mL infusion of cardioplegia, followed by 60 minutes of reperfusion. The cardioplegia consisted of Krebs-Henseleit solution with either vehicle alone (control), 20 mmol KCl, or pinacidil (10, 50, 100, 150, or 200 μmol/L). The developed pressure was measured at baseline and after reperfusion. Coronary blood flow was measured with an in-line ultrasonic probe. Results. Pinacidil (50 μmol/L), as opposed to potassium cardioplegia, provided significantly better postischemic percentage recovery of developed pressure compared with controls (68.3% ± 4.0%, versus 44.6% ± 5.5%; p < 0.05). The time until electrical arrest was significantly shorter in the hyperkalemic group than in all other groups. Linear end-diastolic pressure-volume relationships revealed an increase in slope after ischemia in all groups. Coronary flow after 5 minutes of reperfusion was significantly higher in both the 50-μmol/L and 100-μmol/L pinacidil groups compared with traditional hyperkalemic arrest, and this returned to baseline after 15 minutes. Conclusions. The potassium channel opener pinacidil provided dose-dependent myocardial protection during global ischemia in the blood-perfused rabbit heart model. Potassium-channel openers are a promising class of drugs that may provide an alternative to traditional hyperkalemic cardioplegia.
UR - http://www.scopus.com/inward/record.url?scp=0029942863&partnerID=8YFLogxK
U2 - 10.1016/0003-4975(96)00164-6
DO - 10.1016/0003-4975(96)00164-6
M3 - Article
C2 - 8651767
AN - SCOPUS:0029942863
VL - 61
SP - 1680
EP - 1688
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
SN - 0003-4975
IS - 6
ER -