TY - JOUR
T1 - Myocardial B cells are a subset of circulating lymphocytes with delayed transit through the heart
AU - Adamo, Luigi
AU - Rocha-Resende, Cibele
AU - Lin, Chieh Yu
AU - Evans, Sarah
AU - Williams, Jesse
AU - Dun, Hao
AU - Li, Wenjun
AU - Mpoy, Cedric
AU - Andhey, Prabhakar S.
AU - Rogers, Buck E.
AU - Lavine, Kory
AU - Kreisel, Daniel
AU - Artyomov, Maxim
AU - Randolph, Gwendalyn J.
AU - Mann, Douglas L.
N1 - Publisher Copyright:
© 2020, American Society for Clinical Investigation.
PY - 2020/2/13
Y1 - 2020/2/13
N2 - Current models of B lymphocyte biology posit that B cells continuously recirculate between lymphoid organs, without accumulating in peripheral healthy tissues. Nevertheless, B lymphocytes are one of the most prevalent leukocyte populations in the naive murine heart. To investigate this apparent inconsistency in the literature, we conducted a systematic analysis of myocardial B cell ontogeny, trafficking dynamics, histology, and gene expression patterns. We found that myocardial B cells represent a subpopulation of circulating B cells that make close contact with the microvascular endothelium of the heart and arrest their transit as they pass through the heart. The vast majority (>95%) of myocardial B cells remain intravascular, whereas few (<5%) myocardial B cells cross the endothelium into myocardial tissue. Analyses of mice with B cell deficiency or depletion indicated that B cells modulate the myocardial leukocyte pool composition. Analysis of B cell–deficient animals suggested that B cells modulate myocardial growth and contractility. These results transform our current understanding of B cell recirculation in the naive state and reveal a previously unknown relationship between B cells and myocardial physiology. Further work will be needed to assess the relevance of these findings to other organs.
AB - Current models of B lymphocyte biology posit that B cells continuously recirculate between lymphoid organs, without accumulating in peripheral healthy tissues. Nevertheless, B lymphocytes are one of the most prevalent leukocyte populations in the naive murine heart. To investigate this apparent inconsistency in the literature, we conducted a systematic analysis of myocardial B cell ontogeny, trafficking dynamics, histology, and gene expression patterns. We found that myocardial B cells represent a subpopulation of circulating B cells that make close contact with the microvascular endothelium of the heart and arrest their transit as they pass through the heart. The vast majority (>95%) of myocardial B cells remain intravascular, whereas few (<5%) myocardial B cells cross the endothelium into myocardial tissue. Analyses of mice with B cell deficiency or depletion indicated that B cells modulate the myocardial leukocyte pool composition. Analysis of B cell–deficient animals suggested that B cells modulate myocardial growth and contractility. These results transform our current understanding of B cell recirculation in the naive state and reveal a previously unknown relationship between B cells and myocardial physiology. Further work will be needed to assess the relevance of these findings to other organs.
UR - http://www.scopus.com/inward/record.url?scp=85081654020&partnerID=8YFLogxK
U2 - 10.1172/jci.insight.134700
DO - 10.1172/jci.insight.134700
M3 - Article
C2 - 31945014
AN - SCOPUS:85081654020
SN - 2379-3708
VL - 5
JO - JCI Insight
JF - JCI Insight
IS - 3
M1 - e134700
ER -