Myeloperoxidase produces nitrating oxidants in vivo

Joseph P. Gaut, Jaeman Byun, Hung D. Tran, Jill Roby, Wendy M. Lauber, James A. Carroll, Richard S. Hotchkiss, Abderrazzaq Belaaouaj, Jay W. Heinecke

Research output: Contribution to conferencePaperpeer-review

Abstract

A sensitive and specific gas chromatography/mass spectrometric (GC/MS) technique was developed to measure 3-nitrotyrosine (NT), a marker for reactive nitrogen species (RNS), from tissue samples. Two models of sepsis and measured levels of NT were used to explore the role of myeloperoxidase (MPO), in MPO deficient mice. It was shown that MPO generates reactive nitrogen species by a reaction pathway that required NO 2. The results show that MPO secreted by neutrophils generates 3-nitrotyrosine during acute inflammation under conditions where extracellular levels of NO 2 - and NO 3 - increase markedly.

Original languageEnglish
Pages481-482
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Conference

ConferenceProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period06/2/0206/6/02

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