Myeloperoxidase produces nitrating oxidants in vivo

Joseph P. Gaut; Jaeman Byun; Hung D. Tran; Jill Roby; Wendy M. Lauber; James A. Carroll; Richard S. Hotchkiss; Abderrazzaq Belaaouaj; Jay W. Heinecke

Myeloperoxidase produces nitrating oxidants in vivo

Joseph P. Gaut, Jaeman Byun, Hung D. Tran, Jill Roby, Wendy M. Lauber, James A. Carroll, Richard S. Hotchkiss, Abderrazzaq Belaaouaj, Jay W. Heinecke

Research output: Contribution to conference › Paper › peer-review

Abstract

A sensitive and specific gas chromatography/mass spectrometric (GC/MS) technique was developed to measure 3-nitrotyrosine (NT), a marker for reactive nitrogen species (RNS), from tissue samples. Two models of sepsis and measured levels of NT were used to explore the role of myeloperoxidase (MPO), in MPO deficient mice. It was shown that MPO generates reactive nitrogen species by a reaction pathway that required NO ₂. The results show that MPO secreted by neutrophils generates 3-nitrotyrosine during acute inflammation under conditions where extracellular levels of NO ₂ ^- and NO ₃ ^- increase markedly.

Original language	English
Pages	481-482
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	06/2/02 → 06/6/02

Cite this

@conference{a80e6b152ecf413cbe0a43530b99156a,

title = "Myeloperoxidase produces nitrating oxidants in vivo",

abstract = "A sensitive and specific gas chromatography/mass spectrometric (GC/MS) technique was developed to measure 3-nitrotyrosine (NT), a marker for reactive nitrogen species (RNS), from tissue samples. Two models of sepsis and measured levels of NT were used to explore the role of myeloperoxidase (MPO), in MPO deficient mice. It was shown that MPO generates reactive nitrogen species by a reaction pathway that required NO 2. The results show that MPO secreted by neutrophils generates 3-nitrotyrosine during acute inflammation under conditions where extracellular levels of NO 2 - and NO 3 - increase markedly.",

author = "Gaut, {Joseph P.} and Jaeman Byun and Tran, {Hung D.} and Jill Roby and Lauber, {Wendy M.} and Carroll, {James A.} and Hotchkiss, {Richard S.} and Abderrazzaq Belaaouaj and Heinecke, {Jay W.}",

year = "2002",

language = "English",

pages = "481--482",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Myeloperoxidase produces nitrating oxidants in vivo

AU - Gaut, Joseph P.

AU - Byun, Jaeman

AU - Tran, Hung D.

AU - Roby, Jill

AU - Lauber, Wendy M.

AU - Carroll, James A.

AU - Hotchkiss, Richard S.

AU - Belaaouaj, Abderrazzaq

AU - Heinecke, Jay W.

PY - 2002

Y1 - 2002

N2 - A sensitive and specific gas chromatography/mass spectrometric (GC/MS) technique was developed to measure 3-nitrotyrosine (NT), a marker for reactive nitrogen species (RNS), from tissue samples. Two models of sepsis and measured levels of NT were used to explore the role of myeloperoxidase (MPO), in MPO deficient mice. It was shown that MPO generates reactive nitrogen species by a reaction pathway that required NO 2. The results show that MPO secreted by neutrophils generates 3-nitrotyrosine during acute inflammation under conditions where extracellular levels of NO 2 - and NO 3 - increase markedly.

AB - A sensitive and specific gas chromatography/mass spectrometric (GC/MS) technique was developed to measure 3-nitrotyrosine (NT), a marker for reactive nitrogen species (RNS), from tissue samples. Two models of sepsis and measured levels of NT were used to explore the role of myeloperoxidase (MPO), in MPO deficient mice. It was shown that MPO generates reactive nitrogen species by a reaction pathway that required NO 2. The results show that MPO secreted by neutrophils generates 3-nitrotyrosine during acute inflammation under conditions where extracellular levels of NO 2 - and NO 3 - increase markedly.

UR - http://www.scopus.com/inward/record.url?scp=2442658281&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442658281

SP - 481

EP - 482

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Myeloperoxidase produces nitrating oxidants in vivo

Abstract

Conference

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