Myeloid Conditioning with c-kit-Targeted CAR-T Cells Enables Donor Stem Cell Engraftment

Yasuyuki Arai, Uimook Choi, Cristina I. Corsino, Sherry M. Koontz, Masaki Tajima, Colin L. Sweeney, Mary A. Black, Steven A. Feldman, Mary C. Dinauer, Harry L. Malech

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We report a novel approach to bone marrow (BM) conditioning using c-kit-targeted chimeric antigen receptor T (c-kit CAR-T) cells in mice. Previous reports using anti-c-kit or anti-CD45 antibody linked to a toxin such as saporin have been promising. We developed a distinctly different approach using c-kit CAR-T cells. Initial studies demonstrated in vitro killing of hematopoietic stem cells by c-kit CAR-T cells but poor expansion in vivo and poor migration of CAR-T cells into BM. Pre-treatment of recipient mice with low-dose cyclophosphamide (125 mg/kg) together with CXCR4 transduction in the CAR-T cells enhanced trafficking to and expansion in BM (<1%–13.1%). This resulted in significant depletion of the BM c-kit+ population (9.0%–0.1%). Because congenic Thy1.1 CAR-T cells were used in the Thy1.2-recipient mice, anti-Thy1.1 antibody could be used to deplete CAR-T cells in vivo before donor BM transplant. This achieved 20%–40% multilineage engraftment. We applied this conditioning to achieve an average of 28% correction of chronic granulomatous disease mice by wild-type BM transplant. Our findings provide a proof of concept that c-kit CAR-T cells can achieve effective BM conditioning without chemo-/radiotherapy. Our work also demonstrates that co-expression of a trafficking receptor can enhance targeting of CAR-T cells to a designated tissue. Arai and colleagues demonstrate in a congenic murine transplant model that c-kit-targeted CAR-T cells forced to overexpress CXCR4 showed enhanced trafficking to bone marrow to achieve substantial depletion of endogenous hematopoietic stem cells (HSCs), allowing significant multilineage engraftment of donor HSCs and cure of a chronic granulomatous disease mouse model.

Original languageEnglish
Pages (from-to)1181-1197
Number of pages17
JournalMolecular Therapy
Volume26
Issue number5
DOIs
StatePublished - May 2 2018

Keywords

  • CAR-T cells
  • CXCR4
  • c-kit
  • hematopoietic stem cell transplantation
  • immunotherapy

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