@article{d7d6e6f4a0d74e0aaf370094cf8a9967,
title = "Mycobacterium tuberculosis infection drives a type I IFN signature in lung lymphocytes",
abstract = "Mycobacterium tuberculosis (Mtb) infects 25% of the world's population and causes tuberculosis (TB), which is a leading cause of death globally. A clear understanding of the dynamics of immune response at the cellular level is crucial to design better strategies to control TB. We use the single-cell RNA sequencing approach on lung lymphocytes derived from healthy and Mtb-infected mice. Our results show the enrichment of the type I IFN signature among the lymphoid cell clusters, as well as heat shock responses in natural killer (NK) cells from Mtb-infected mice lungs. We identify Ly6A as a lymphoid cell activation marker and validate its upregulation in activated lymphoid cells following infection. The cross-analysis of the type I IFN signature in human TB-infected peripheral blood samples further validates our results. These findings contribute toward understanding and characterizing the transcriptional parameters at a single-cell depth in a highly relevant and reproducible mouse model of TB.",
keywords = "CP: Immunology, CP: Microbiology, Mycobacterium tuberculosis, lymphocytes, single-cell RNA sequencing, type I IFN",
author = "Sadia Akter and Chauhan, {Kuldeep S.} and Dunlap, {Micah D.} and Chore{\~n}o-Parra, {Jos{\'e} Alberto} and Lan Lu and Ekaterina Esaulova and Joaquin Z{\'u}{\~n}iga and Artyomov, {Maxim N.} and Deepak Kaushal and Khader, {Shabaana A.}",
note = "Funding Information: This work was supported by Washington University in St. Louis School of Medicine and NIH grants HL105427, AI111914, AI134236, AI155024, and AI123780 to S.A.K. and D.K. The authors wish to thank Amanda Swain, Washington University School of Medicine in St. Louis, for the biological data collection and Leah Mellett for helping in graphical abstract image creation with Biorender.com. Conceptualization, D.K. and S.A.K.; investigation, S.A. K.S.C. M.D.D. J.A.C.-P. E.E. J.Z. M.N.A. D.K. and S.A.K.; formal analysis and data curation, S.A. K.S.C. D.K. and S.A.K.; visualization, S.A. and K.S.C; writing – original draft, S.A. K.S.C. D.K. and S.A.K.; writing – review & editing, S.A. K.S.C. D.K. and S.A.K.; funding acquisition, D.K. and S.A.K.; supervision, D.K. and S.A.K. The authors declare no competing interests. One or more of the authors of this paper self-identifies as an underrepresented ethnic minority in science. The author list of this paper includes contributors from the location where the research was conducted who participated in the data collection, design, analysis, and/or interpretation of the work. Funding Information: This work was supported by Washington University in St. Louis School of Medicine and NIH grants HL105427 , AI111914 , AI134236 , AI155024 , and AI123780 to S.A.K. and D.K. The authors wish to thank Amanda Swain, Washington University School of Medicine in St. Louis, for the biological data collection and Leah Mellett for helping in graphical abstract image creation with Biorender.com . Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = jun,
day = "21",
doi = "10.1016/j.celrep.2022.110983",
language = "English",
volume = "39",
journal = "Cell Reports",
issn = "2211-1247",
number = "12",
}