Mycobacterium tuberculosis infection drives a type I IFN signature in lung lymphocytes

Sadia Akter, Kuldeep S. Chauhan, Micah D. Dunlap, José Alberto Choreño-Parra, Lan Lu, Ekaterina Esaulova, Joaquin Zúñiga, Maxim N. Artyomov, Deepak Kaushal, Shabaana A. Khader

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Mycobacterium tuberculosis (Mtb) infects 25% of the world's population and causes tuberculosis (TB), which is a leading cause of death globally. A clear understanding of the dynamics of immune response at the cellular level is crucial to design better strategies to control TB. We use the single-cell RNA sequencing approach on lung lymphocytes derived from healthy and Mtb-infected mice. Our results show the enrichment of the type I IFN signature among the lymphoid cell clusters, as well as heat shock responses in natural killer (NK) cells from Mtb-infected mice lungs. We identify Ly6A as a lymphoid cell activation marker and validate its upregulation in activated lymphoid cells following infection. The cross-analysis of the type I IFN signature in human TB-infected peripheral blood samples further validates our results. These findings contribute toward understanding and characterizing the transcriptional parameters at a single-cell depth in a highly relevant and reproducible mouse model of TB.

Original languageEnglish
Article number110983
JournalCell Reports
Volume39
Issue number12
DOIs
StatePublished - Jun 21 2022

Keywords

  • CP: Immunology
  • CP: Microbiology
  • Mycobacterium tuberculosis
  • lymphocytes
  • single-cell RNA sequencing
  • type I IFN

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